| 哺乳动物Y染色体的测序进展 |
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| 引用本文： | 滑留帅,王璟,汪聪勇,师志海,施巧婷,王二耀.哺乳动物Y染色体的测序进展[J].中国草食动物科学,2021(2). |
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| 作者姓名： | 滑留帅 王璟 汪聪勇 师志海 施巧婷 王二耀 |
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| 作者单位： | 河南省农业科学院畜牧兽医研究所;河南省鼎元种牛育种有限公司;河南省畜禽繁育与营养调控重点实验室;郑州市兽医生物信息重点实验室 |
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| 基金项目： | 河南省农业科学院自主创新专项基金(2020ZC37);河南省肉牛产业技术体系(S2013-08);国家肉牛牦牛产业技术体系(CARS-37)。 |
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| 摘 要： | 哺乳动物的性染色体由一对常染色体演化而来,其中X染色体在物种间相对保守,而Y染色体则存在很大的变异,包括染色体的大小、结构和基因数量等。研究Y染色体的遗传结构与变异,对于理解哺乳动物的起源进化、性别决定以及动物繁殖都具有重要意义。因此,文章综述了哺乳动物Y染色体的结构与变异,以及Sanger测序技术、二代测序技术、三代测序技术在Y染色体测序中的应用,并展望了基于CRISPR-dCas9可视化系统的流式染色体分离技术,以及高精度的三代测序技术在Y染色体测序中的应用前景。
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| 关 键 词： | Y染色体 测序 CRISPR-dCas9 PacBio CCS |
Sequencing Progress of Mammalian Y Chromosome |
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| HUA Liushuai,WANG Jing,WANG Congyong,SHI Zhihai,SHI Qiaoting,WANG Eryao.Sequencing Progress of Mammalian Y Chromosome[J].China Herbivores,2021(2). |
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| Authors: | HUA Liushuai WANG Jing WANG Congyong SHI Zhihai SHI Qiaoting WANG Eryao |
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| Institution: | (Institute of Animal Husbandry and Veterinary Science,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Henan Dingyuan Cattle Breeding Co.Ltd.,Zhengzhou 450046,China;Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation,Zhengzhou 450002,China;Zhengzhou Key Laboratory of Veterinary Bioinformatics,Zhengzhou 450002,China) |
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| Abstract: | The sex chromosomes of mammals evolved from a pair of autosomes.The X chromosome is relatively conserved among species,while the Y chromosome has great variations,including the size,structure,and number of genes.Studying the genetic structure and variation of the Y chromosome is of great significance for understanding the origin and evolution of mammals,sex determination,and animal reproduction.The structure and variation of mammalian Y chromosome,and the application of Sanger sequencing technology,second-generation sequencing technology,and third-generation sequencing technology in Y chromosome sequencing was reviewed.The application prospects of flow chromosome separation technology based on the CRISPR visualization system and the high-precision third-generation sequencing technology in Y chromosome sequencing were reviewed. |
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| Keywords: | Y chromosome sequencing CRISPR-dCas9 PacBio CCS |
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