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| 荧光定量PCR在高致病性猪蓝耳病检测上的应用 | |
| 引用本文： | 曹火仁,顾晓峰,朱骏,沈林辉,孙文梅,曹向英.荧光定量PCR在高致病性猪蓝耳病检测上的应用[J].四川畜牧兽医,2010,37(6):21-22,25. |
| 作者姓名： | 曹火仁 顾晓峰 朱骏 沈林辉 孙文梅 曹向英 |
| 作者单位： | 1. 上海市松江区动物疫病预防控制中心,上海,201611 2. 上海交通大学农业与生物学院,上海,200240 3. 上海市松江区食用农产品安全监督检测中心,上海,201611 |
| 摘 要： | 应用实时荧光定量RT-PCR方法对上海松江区8个生猪重点养殖地区的60份样品（血液样品50份、组织病料10份）,以及10个规模化猪场的100份样品（血液样品81份、组织病料19份）进行高致病性猪蓝耳病的检测,结果均为阴性,表明该区猪群中没有高致病性猪蓝耳病的隐性感染和健康带毒。同时,熟悉并完善了高致病性猪蓝耳病的荧光定量RT-PCR检测技术。 |
| 关 键 词： | 高致病性猪蓝耳病 实时荧光定量RT-PCR |
Application of fluorescence quantitative PCR in the detection of highly pathogenic PRRS |
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| Institution: | CAO Huo-ren,GU Xiao-feng,ZHU Jun,et al. (1.Center for Animal Disease Control and Prevention in Songjiang District,Shanghai 201611,China; 2.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China; 3.Food Safety Supervision and Inspection Center of Agricultural Products in Songjiang District,Shanghai 201611,China) |
| Abstract: | The real -time fluorescence quantitative RT -PCR was applied to detect the highly pathogenic PRRSV in 8 pig breeding areas and 10 pig farms. 60 samples,including 50 blood samples and 10 tissue samples,which were collected from the 8 pig breeding areas in Songjiang District. At the same time,81 blood samples and 19 tissue samples were collected from the other 10 pig farms. The results showed that highly pathogenic PRRSV was never detected in the samples, which could indicate that latent infection of highly pathogenic PRRSV and healthy carrier didn＇t take place in our district. In addition,the real-time fluorescence quantitative RT-PCR for highly pathogenic PRRSV was established and optimized. |
| Keywords: | Highly pathogenic PRRS Real-time fluorescence quantitative PCR |
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