| 重庆某地仔猪免疫蓝耳病活疫苗的最适剂量筛选 |
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| 引用本文： | 胡昌金,陈勇,段兵,唐臣学,程绍伟,范才良.重庆某地仔猪免疫蓝耳病活疫苗的最适剂量筛选[J].四川畜牧兽医,2014(11):27-30. |
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| 作者姓名： | 胡昌金 陈勇 段兵 唐臣学 程绍伟 范才良 |
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| 作者单位： | 1. 重庆市荣昌县畜牧兽医局,重庆 荣昌,402460
2. 西南大学荣昌校区动物医学系,重庆 荣昌,402460 |
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| 摘 要： | 本试验采用1、2、4头份免疫剂量对重庆市荣昌县不同地区的仔猪进行PRRS免疫,然后在免疫后28 d和60 d分别采取血清用ELISA检测免疫抗体水平。结果显示:免疫4头份的抗体阳性率最高,为100%;其次为2头份免疫,抗体阳性率为91.67%;而1头份免疫的抗体阳性率仅为50%。进一步测定抗体滴度,发现2头份、4头份剂量在28 d和60 d的抗体效果差异性不显著。综合考虑,注射2头份高致病性猪蓝耳病活疫苗的免疫效果最佳。
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| 关 键 词： | 高致病性猪蓝耳病 活疫苗 免疫抗体 检测 仔猪 |
Screening of Optimum Dosage of PRRS Vacine on Piglets in Chongqing |
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| Institution: | HU Changjin, CHEN Yong, CHENG Shaowei, et al. (1.Animal Husbandry and Veterinary Bureau of Rongchang County, Chongqing Rongchang 402460 2.Veterinary Medicine Department, Rongchang Campus of Southwest University, Chongqing Rongchang 402460, China ) |
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| Abstract: | In this experiment, the antibody level of piglet in different farm immunized with PRRSV live vaccine （1 dose,2 dose and 4 dose） were detected by method of enzyme-linked im-muno sorbent assay （ELISA） at 28 days and 60 days after immunization. The results demonstrated that the highest antibody positive rate （100%） was found in 4 dose group,followed by 2 dose （an-tibody positive rate was 91.67%） and 1 dose （50%）. There wasn′t significant difference between im-mune dosage of 2 and 4 with PRRSV live vaccine at 28 days and 60 days later. The experimental result indicated that the optimum immune dosage of PRRSV live vaccine was 2 doage. |
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| Keywords: | PRRS Live vaccine Immunization antibody Detection Piglet |
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