| 鲫鱼弗氏柠檬酸杆菌的耐药表型及耐药基因检测 |
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| 引用本文： | 王利,魏勇,龙钟明,刘港彪.鲫鱼弗氏柠檬酸杆菌的耐药表型及耐药基因检测[J].黑龙江畜牧兽医,2012(15):14-16. |
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| 作者姓名： | 王利 魏勇 龙钟明 刘港彪 |
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| 作者单位： | 西南民族大学生命科学与技术学院动物遗传育种学国家民委-教育部重点实验室;四川省畜牧科学研究院 |
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| 基金项目： | 四川省科技厅应用基础项目(2011JY0095);西南民族大学研究生学位点建设项目(2011XWD-S071007) |
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| 摘 要： | 为了检测鲫鱼费氏柠檬酸杆菌的耐药表型及耐药基因,试验采用标准Kirby-Bauer纸片扩散法检测鲫鱼弗氏柠檬酸杆菌对13种抗生素的耐药表型,并用PCR法检测弗氏柠檬酸杆菌对四环素类和磺胺类抗生素的6种耐药基因(tetA、tetC、tetM、Sul1、Sul2和Sul3)。结果表明:鲫鱼弗氏柠檬酸杆菌对四环素、氨苄西林、强力霉素、红霉素、头孢拉定和阿莫西林耐药;仅检测出四环素类的tetA和tetM基因,而其他未检测出。
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| 关 键 词： | 弗氏柠檬酸杆菌 耐药基因 耐药表型 鲫鱼 |
Detection of the drug-resistant phenotypes and genes in Citrobacter freundii isolated from Carassius aumtus |
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| WANG Li,WEI Yong,LONG Zhong-ming,LIU Gang-biao.Detection of the drug-resistant phenotypes and genes in Citrobacter freundii isolated from Carassius aumtus[J].Heilongjiang Animal Science And veterinary Medicine,2012(15):14-16. |
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| Authors: | WANG Li WEI Yong LONG Zhong-ming LIU Gang-biao |
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| Institution: | 1(1.Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education,College of Life Science and Technology,Southwest University for Nationalities, Chengdu 610041,China;2.Sichuan Academy of Animal Science,Chengdu 610066,China) |
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| Abstract: | To detect the drug-resistant phenotypes and genes in Citrobacter freundii isolated from Carassius aumtus,the standard Kirby Bauer disc diffusion method was used to detect the drug-resistant phenotypes of Citrobacter freundii isolated from Carassius aumtus against 13 kinds of antibiotics,and the PCR method was also used to detect the six kinds of drug-resistant genes including tetA,tetC,tetM,Sul1,Sul2 and Sul3 genes against tetracyclines and sulfonamides antibiotics.The results showed that the Citrobacter freundii was resistant to tetracycline,ampicillin,doxycycline,erythromycin,cefradine and amoxicillin.Only the tetA and tetM genes against tetracyclines were checked out,while the other drug-resistant genes were not detected in the experiment. |
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| Keywords: | Citrobacter freundii drug-resistant gene drug-resistant phenotype Carassius aumtus |
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