非繁殖季节中国美利奴羊血清E2、P4、FSH、LH激素变化规律研究

为了在非繁殖季节判断绵羊的发情状况,试验以新疆高海拔地区中国美利奴羊为研究对象,采用酶联免疫测定(ELISA)法测定绵羊血清中雌二醇(E2)、孕酮(P4)、促卵泡素(FSH)、促黄体生成素(LH)的含量,研究激素诱导发情羊、发情症状不明显羊、不发情羊体内激素分泌规律的差异。结果表明:激素诱导发情羊的E2含量比不发情羊高,差异显著(P<0.05);但是在P4、FSH、LH水平上,激素诱导发情羊要低于不发情羊,差异不显著(P>0.05)。发情羊与发情不明显羊在4种激素含量上差异不显著(P>0.05),说明公羊试情存在一定缺陷,而通过测定E2含量判定绵羊发情状态将成为未来研究的重点。

The study on the variation laws for E2,P4,FSH and LH in serum from Chinese Merino sheep during the non-breeding season

During non-breeding season,the Chinese Merino sheep at high altitude in Xinjiang were chosen as the experimental objects.The variation in content of E2,P4,FSH and LH were measured by enzyme-linked immunosorbent assay(ELISA),the secretion differences of the four kinds of hormone among estrus sheep,anestrus sheep,and sheep with indistinct estrus symptoms were studied.The results showed that the estradiol(E2) level of hormone-induced estrus sheep was higher than that of anestrus sheep,and the difference was significant(P<0.05),but hormone-induced estrus sheep was lower than anestrus sheep in P4,FSH and LH levels,and the difference was not significant(P>0.05).There were no significant differences between estrus sheep and sheep with indistinct estrus symptoms in E2,P4,FSH and LH levels(P>0.05).It indicated that there were some flaws in ram test.However,determining the estrus states in sheep will be the focus of future research through measuring E2 levels.