| 牛结核分枝杆菌LppA基因克隆与序列分析 |
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| 引用本文： | 徐广贤,李娟,李勇,王玉炯.牛结核分枝杆菌LppA基因克隆与序列分析[J].黑龙江畜牧兽医,2009(5). |
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| 作者姓名： | 徐广贤 李娟 李勇 王玉炯 |
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| 作者单位： | 宁夏大学 生命科学学院,宁夏,银川,750021
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| 基金项目： | 国家重点基础研究发展规划(973计划)，高等学校科技创新工程重大项目培育资金，宁夏大学科研基金 |
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| 摘 要： | 牛结核分枝杆菌LppA基因是一个编码磷脂蛋白的基因,为了研究牛结核分枝杆菌LppA基因的多态性,试验以牛结核分枝杆菌基因组DNA为模板,克隆 LppA基因,并进行序列测定,然后利用DNAMAN软件对其序列进行分析.结果表明:LppA基因全长564 bp,编码187个氨基酸,与GenBank所发表的牛结核分枝杆菌LppA基因的核苷酸同源性为99.47% ,氨基酸同源性为98.40%,有3处位点发生有义突变.
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| 关 键 词： | 牛结核分枝杆菌 序列分析 |
The LppA gene cloning and sequence analysis of Mycobacterium bovis |
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| XU Guang-xian,LI Juan,WANG Yu-jiong,et al.The LppA gene cloning and sequence analysis of Mycobacterium bovis[J].Heilongjiang Animal Science And veterinary Medicine,2009(5). |
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| Authors: | XU Guang-xian LI Juan WANG Yu-jiong |
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| Institution: | College of Life Science;Ningxia University;Yinchuan 750021;China |
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| Abstract: | The LppA gene encods the lipoprotein.In order to study the LppA polymorphism of the Mycobacterium bovis,a PCR strategy was performed by using M.bovis DNA as the template and then amplifying LppA genes,which was cloned into pMD18-T and sequenced,then the nucleotide sequence of positive recon was analyzed by DNAMAN,the results showed that the total length of LppA was 564 bp,encoded 187 amino acids;compared with LppA sequence published on GenBank,the nucleotide homology was 99.47%,while the amino acid homology... |
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| Keywords: | LppA |
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