| 牛传染性支气管炎病毒PCR诊断方法的研究 |
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| 引用本文： | 张桂红,童光志,王柳,仇华吉,赵晓岩,张绍杰,刘宝全.牛传染性支气管炎病毒PCR诊断方法的研究[J].黑龙江畜牧兽医,2000(4):1-2. |
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| 作者姓名： | 张桂红 童光志 王柳 仇华吉 赵晓岩 张绍杰 刘宝全 |
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| 作者单位： | [1]东北农业大学动物医学院,黑龙江哈尔滨 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨 |
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| 基金项目： | 黑龙江省农业现代化创新工程资助项目，， |
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| 摘 要： | 根据已发表的牛传染性鼻气管炎病毒（ＩＢＲＶ）ｇＢ基因序列，应用ｏｌｉｇｏ４．１程序设计一对引物ＰＢ１／ＰＢ２，分别对ＩＢＲＶＬＡ株、洛精、美精、ＢａｒｔｈａＮｕ／６７株、Ｂ７株、云南－１和云南－２分离株进行ＰＣＲ扩增。结果显示７株ＩＢＲ病毒ＤＮＡ均有３９９ｂｐ的特异性片段。用此引物对其同属的伪狂犬病毒（ＰＲＶ）、马立克氏病毒（ＭＤＶ）、鸡传染必喉气管炎病毒（ＩＬＴＶ）进行扩增。均未见特异性条带，证
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| 关 键 词： | 牛 传染性鼻气管炎病毒 gB基因 PCR诊断方法 |
| 文章编号： | 1004-7034(2000)04-0001-02 |
| 修稿时间： | 1999年9月23日 |
Study on a PCR Method for Diagnosis of Bovine Infectious Rhinotra cheitis Virus |
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| ZHANG Gui-hong,TONG Guang-zhi,WANG Liu,QIU Hua-ji,ZHAO Xiao-yan,ZHANG Shao-jie,LIU Bao-quan.Study on a PCR Method for Diagnosis of Bovine Infectious Rhinotra cheitis Virus[J].Heilongjiang Animal Science And veterinary Medicine,2000(4):1-2. |
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| Authors: | ZHANG Gui-hong TONG Guang-zhi WANG Liu QIU Hua-ji ZHAO Xiao-yan ZHANG Shao-jie LIU Bao-quan |
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| Abstract: | One pairs of primers PB1/PB2 were designed using oligo 4.1 program based on the published sequences of gB genes of IBRV.The DNA sextracted from the IBRV strains LA,Luojing,Meijing,Bartha Nu/67,B7,Yunnan-1 and Yunnan-2 were subjected to amplifying by PCR using the primers.The amplification yielded a specific band of 339 bp for all the IBRVs when using PB1/PB2.There were no specific amplifications for pseudorabies(PRV),Marek' disease virus(MDV) and infectious laryngotracheitis virus(ILTV).The PCR was shown to be sensitive and could detect as low as 3 pgs of viral DNAs. |
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| Keywords: | Infectious bovine rhinotracheitis virus(IBRV) gB gene PCR method |
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