中草药对奶牛乳房炎致病菌的体外抑菌效果试验

目的 为研究中草药对奶牛乳房炎主要致病菌体外抑菌效果。方法 试验选取金银花等10 味中草药,采用试管二倍稀释法测定其对金黄色葡萄球菌、大肠杆菌和无乳链球菌的最低抑菌浓度（MIC）。结果 中草药对主要致病菌有不同程度的抑菌效果。连翘对金黄色葡萄球菌的抑菌效果最好,MIC为15.63 mg/mL;板蓝根、蒲公英和黄芩对大肠杆菌抑菌效果最好,MIC为62.5 mg/mL;紫花丁对无乳链球菌抑菌效果最好,MIC为15.63 mg/mL。结论 试验为临床研制乳房炎中草药制剂提供了技术参考。

In vitro Antibacterial Effect of Chinese Herbal Medicine on Pathogenic Bacteria of Dairy Cow Mastitis

Objective] In order to study the in vitro antibacterial effects of Chinese herbal medicines on main pathogenic bacteria of dairy cow mastitis. Method] 10 Chinese herbal medicines such as Honeysuckle were selected to determine the minimum antibacterial concentrations of Staphylococcus aureus,Escherichia coli and Streptococus agalactiae by test tube two-fold dilution method. Result] The Chinese herbal medicine had different degree of bacteriostatic effect on main pathogenic bacteria. Forsythia had the best antibacterial effect on Staphylococcus aureus,with MIC of 15.63 mg/mL. Radixisatidis,Dandelion and Scutellaria baicalensis had the he best antibacterial effect on Escherichia coli,with MIC of 62.5mg/mL. Lilac had the he best antibacterial effect on Streptococus agalactiae,with MIC of 15.63 mg/mL.Conclusion] This study provided technical reference for clinical development of Chinese herbal medicine mastitis preparation.