Serological update and molecular characterization of Dirofilaria immitis in dogs,South Korea |
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| Authors: | KH Song JE Park DH Lee SH Lee HJ Shin |
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| Institution: | 1. Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries, Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang City 420-480, Republic of Korea;2. Immunology Laboratory, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam;3. Dept. of Botany, Plant Molecular Biology Laboratory, S. K. University, Anantapur, Andhrapradesh, India;4. Systemic Disease Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang City 420-480, Republic of Korea;5. Bacterial Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang City 420-480, Republic of Korea;6. Dept. of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang City 420-480, Republic of Korea;1. Department of Microbiology, Konkuk University School of Medicine, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea;2. Research Institute of Medical Science, Konkuk University School of Medicine, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea;3. Force Health Protection and Preventive Medicine, Medical Department Activity-Korea/65th Medical Brigade, Unit 15281, APO AP 96271-5281, USA;4. Viral and Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910, USA;5. Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA |
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| Abstract: | Eighty-one dogs in the Chungnam province were tested for heartworm (Dirofilaria immitis) infection by ELISA (SNAP test, IDEXX Laboratories, Maine, USA). Seventeen (20.9%) of the 81 samples were found to be positive and further analyzed by 16S rRNA sequencing. In this study, all dogs tested lived outdoors. Using the χ2 test and Fisher’s exact test, no significant differences in the prevalence of dirofilariasis were observed among different gender and age groups, although the prevalence of this disease among dogs 2–4 yrs of age remains highest. Sequence analysis revealed that the species prevalent in Chungnam province were genetically distinct from the type strain of D. immitis based on the nucleotide deletion found at position nt 276 (cytosine) and nucleotide substitution at position 428 (G to A) of the partial 16S rRNA sequence of the type strain. Furthermore, phylogenetic analysis suggests at least 2 groups of D. immitis circulating in the Chungnam area between the year 2007 and 2008. |
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