Phylogenetic distribution of virulence genes in Escherichia coli isolated from bovine mastitis in Iran |
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| Authors: | Reza Ghanbarpour Eric Oswald |
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| Institution: | a Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O. Box 76169, 14111 Kerman, Iran
b UMR 1225 INRA-ENVT Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire, 23 chemin des Capelles BP 87614, 31076 Toulouse Cedex 3, France |
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| Abstract: | One hundred and twenty seven Escherichia coli isolates from bovine mastitis were examined to detect the phylogenetic group/subgroups and a selection of virulence associated genes. Forty nine (38.58%) isolates belonged to group B1 the remaining isolates fell into four phylogenetic subgroups: A0 (18.11%), A1 (26.77%), D1 (6.29%) and D2 (10.23%). None of the isolates belonged to B2 group. Forty seven (37.00%) isolates were positive for at least one virulence gene, among them f17A was the most common gene, found in 20.47% of the isolates. Among the E. coli isolates, 11.81% had iucD, 9.44% f17c-A, 9.44% cnf2, 7.87% f17b-A, 6.29% afaD-8 and afaE-8, 3.14% f17d-A, 0.78% cnf1 and 0.78% clpG genes. All of the detected virulence genes were present alone or in combination with each other except clpG and f17d-A genes that were only found alone. None of the isolates contained the genes for F17a-A, intimin, P or S fimbriae. |
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| Keywords: | Mastitis Escherichia coli Phylogenetic group Virulence gene Cow |
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