| 南方型紫花苜蓿耐盐细胞系的筛选及生理特性分析 |
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| 引用本文： | 马进,刘志高,郑钢.南方型紫花苜蓿耐盐细胞系的筛选及生理特性分析[J].中国草地学报,2011,33(4). |
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| 作者姓名： | 马进 刘志高 郑钢 |
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| 作者单位： | 浙江农林大学园林学院,浙江临安,311300 |
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| 基金项目： | 浙江省自然科学基金“耐南方滩涂种植的紫花苜蓿细胞突变体筛选及机理研究”(Y304072)、“南方型紫花苜蓿耐盐突变体盐胁迫特异蛋白及相关基因筛选与鉴定”(Y3090498); 国家“十二五”科技支撑计划项目“中东部地区优质紫花苜蓿高效生产利用关键技术研究与集成示范”(2011BAD17B04) |
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| 摘 要： | 以NaCl为选择因子,对长期继代培养的紫花苜蓿愈伤组织进行选择,筛选出耐1.2%NaCl的耐盐细胞系并对其进行生理分析,结果表明:耐盐细胞系对逆境的忍受能力高于对照,积累的脯氨酸和可溶性蛋白含量也高于对照,维持着较高的K+/Na+比,在盐分胁迫下能维持较高的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。
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| 关 键 词： | 南方型紫花苜蓿 耐盐细胞系 筛选 生理特性 |
Selection and Physiological Characterization of Salt-tolerant Cell Line of Southern Type Alfalfa |
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| MA Jin,LIU Zhi-gao,ZHENG Gang.Selection and Physiological Characterization of Salt-tolerant Cell Line of Southern Type Alfalfa[J].Chinese Journal of Grassland,2011,33(4). |
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| Authors: | MA Jin LIU Zhi-gao ZHENG Gang |
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| Institution: | MA Jin,LIU Zhi-gao,ZHENG Gang (Faculty of Landscape Architecture,Zhejiang Agriculture and Forestry University,Lin'an 311300,China) |
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| Abstract: | Calluses of Southern type alfalfa cultured for a long term were selected,a cell line tolerant to 1.2% NaCl was isolated by using NaCl as selection pressure.The physiological and biochemical properties of this salt tolerant cell line were analyzed.The results showed that the tolerance of the salt-tolerant cell line was much higher than that of control line.Free proline content and soluble protein content increased in salt-tolerant cell line was higher than that in control,a higher K+/Na+ ratio was kept in th... |
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| Keywords: | Southern type Medicago sativa Salt-tolerance cell line Selection Physiological characterization |
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