| 基质辅助激光解析电离飞行时间质谱在动物病原菌检测中的应用 |
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| 引用本文： | 龚艳清,郭书林,陈信忠,杨俊萍,马群飞,叶妍妍.基质辅助激光解析电离飞行时间质谱在动物病原菌检测中的应用[J].动物检疫,2012(2):22-25. |
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| 作者姓名： | 龚艳清 郭书林 陈信忠 杨俊萍 马群飞 叶妍妍 |
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| 作者单位： | [1]厦门出入境检验检疫局,福建厦门361026 [2]福建省疾病预防控制中心,福建福州350002 |
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| 基金项目： | 福建省社会发展重点项目（2010Y0049）资助 |
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| 摘 要： | 应用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）技术,对厦门口岸进口的1000多份动物及其产品的样品进行致病菌的检测和鉴定,共检出20多种致病菌,并对这些菌株同时用传统生化鉴定方法进行确认。结果表明MALDI-TOF-MS对未知细菌进行鉴定,较传统方法更加快速、准确,而且可以进行高通量检测,可以广泛应用于口岸动物检疫以及微生物检验实验室的日常检验。此外,将国内分离鉴定的各种参考菌株建立的常见致病菌MALDI-TOF-MS数据库与布鲁克公司的MALDI Biotyper数据库进行病原菌鉴定的比较,结果表明,实验室自建的MALDI-TOF-MS数据库可以取得更加准确地结果。
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| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱 动物致病菌 检测 |
Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Detection of Animal Pathogenic Bacteria |
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| Authors: | Gong Yanqing Guo Shulin Chen Xinzhong Yang Junping Ma Qunfei Ye Yanyan |
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| Institution: | 1. Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361012; 2. Fujian Center for Disease Control and Prevention,Fuzhou 350002) |
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| Abstract: | More than 1 000 animals and animal products imported via Xiamen port were detected and identified for pathogenic bacteria by Matrix-assisted laser desorption ionization-time of flight mass spectrometry（MALDI-TOF MS）. More than 20 pathogenic bacteria were identified and then confirmed by conventional techniques. The results showed that compared with the conventional techniques,MALDI-TOF MS was more convenient,reliable and high-through. It can be used for port animal quarantine inspection and daily quarantine diagnosis in microbiological laboratories. Furthermore,a comparison showed that the MALDI-TOF MS database of pathogenic bacteria built by referencing bacteria cultured in our country can offer more reliable results than the MALDI Biotyper database by Bruker Daltonik company. |
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| Keywords: | Matrix-assisted laser desorption ionization–time of flight mass spectrometry（MALDI-TOF MS） animal pathogenic bacteria detection identification |
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