| 根癌农杆菌介导AtNHX1基因转化紫花苜蓿的研究 |
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| 引用本文： | 王强龙,王锁民,张金林,包爱科,陈托兄,楼洁琼,陆妮.根癌农杆菌介导AtNHX1基因转化紫花苜蓿的研究[J].草业科学,2006,23(12):55-59. |
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| 作者姓名： | 王强龙 王锁民 张金林 包爱科 陈托兄 楼洁琼 陆妮 |
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| 作者单位： | 兰州大学草地农业科技学院,甘肃,兰州,730000;内江师范学院,四川,内江,641112;兰州大学草地农业科技学院,甘肃,兰州,730000 |
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| 基金项目： | 国家自然科学基金，教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 在已建立的紫花苜蓿Medicogo sativa体细胞胚高频再生体系的基础上,研究了OD600、侵染时间、负压处理、预培养及共培养时间、脱菌及选择方法等因子对转化效率的影响,并初步获得了抗性体胚。
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| 关 键 词： | 紫花苜蓿 体细胞胚 根癌农杆菌 AtNHX1 遗传转化 |
| 文章编号： | 1001-0629（2006）12-0055-05 |
| 收稿时间： | 03 6 2006 12:00AM |
| 修稿时间： | 2006-03-06 |
Transformation studies of Medicago sativa mediated by Agrobacterium tumefaciens with AtNHX1 gene |
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| WANG Qiang-long,WANG Suo-min,ZHANG Jin-lin,BAO Ai-ke,CHEN Tuo-Xiong,LOU Jie-qiong,LU Ni.Transformation studies of Medicago sativa mediated by Agrobacterium tumefaciens with AtNHX1 gene[J].Pratacultural Science,2006,23(12):55-59. |
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| Authors: | WANG Qiang-long WANG Suo-min ZHANG Jin-lin BAO Ai-ke CHEN Tuo-Xiong LOU Jie-qiong LU Ni |
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| Institution: | 1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730000, China ; 2. Neijiang Teachers college, Neijiang 641112, China |
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| Abstract: | Several factors (bacterial concentrations, infected time, negative pressure treatment time, pre-culture and co-culture time, acterium-free and screening methods etc. ) affecting genetic transformation of alfalfa (Medicago sativa), which is mediated by Agrobacterium tumefaciens with AtNHX1 gene, were studied on the basis of high-frequency regeneration system of alfalfa somatic embryos. Transformation system for alfalfa was successfully set up and the transgenic somatic embryos were also obtained. |
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| Keywords: | Medicago saliva somatic embryos Agrobacterium tumefaciens AtNHX1 genetic transformation |
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