"兰箭3号"春箭筈豌豆叶绿体全基因组草图及特征分析

箭筈豌豆(Vicia sativa)是自花授粉的二倍体一年生豆科牧草,可为我国高海拔地区的反刍动物提供优质蛋白粗饲料。以"兰箭3号"春箭筈豌豆为研究对象,采用DNase I法纯化叶绿体,利用第二代高通量测序平台Illumina Hiseq2000进行测序,并对"兰箭3号"叶绿体全基因组序列的结构特征进行分析。结果表明,"兰箭3号"叶绿体基因组仅包括一个单拷贝的反向重复序列,其叶绿体全基因组大小为121 883bp,共编码了109个基因,包括4个核糖体RNA(rRNA)基因,29个转运RNA(tRNA)基因,75个蛋白质编码基因和1个假基因。"兰箭3号"在叶绿体基因组结构、基因种类、排列顺序上与其它豆科植物基本一致。其叶绿体全基因组序列已在GenBank注册,序列号为KU053796。"兰箭3号"叶绿体全基因组测序的成功为叶绿体分子生物学研究奠定了基础,并可有效地促进箭筈豌豆遗传育种和分子进化研究。

The draft and characterization of the complete chloroplast genome of Vicia sativa cv.Lanjian No.3

The common vetch (Vicia sativa) is a self-pollinating and diploid annual legume, which provides high-quality protein roughage for ruminants of high altitude areas in China.In this study, we evaluated the V.sativa cv.Lanjian No.3 using Illumina Hiseq 2000 to analyze the chloroplast genome sequence.The results showed that this cultivar chloroplast genome is 121 883 bp in length with only one copy of the IR region.It encodes 109 genes, including 4 ribosomal RNA (rRNA) genes, 29 transfer RNA (tRNA) genes, 75 protein-coding genes, and 1 pseudogene.Compared with other legume species, V.sativa chloroplast genes have few differences in size, order, and structure.This genome sequence has been registered in GenBank under accession number KU053796.The successful sequencing of this genome contributes to chloroplast molecular biology and will be useful in studying genetic breeding and molecular evolution of the common vetch.