黄帚橐吾乙醇提取物对小鼠的急性毒性试验

黄帚橐吾(Ligularia virgaurea)是一种在甘南草原上广泛分布的毒杂草,但同时其乙醇提取物有很强的生物农药活性,具备开发成为生物农药的潜力。本研究通过昆明小鼠(Mus musculus)的经皮、经口急性毒性试验对黄帚橐吾乙醇提取物的安全性进行评估。结果显示,黄帚橐吾乙醇提取物对小鼠的经皮急性毒性半致死剂量LD5030g·kg-1,经口急性毒性LD50为6.983 1g·kg-1,均符合低毒农药登记的毒性标准,可以按照低毒农药进行开发应用。

Toxicity of ethanol extracts from Ligularia virgaurea

Ligularia virgaurea,is a widespread poisonous plant species growing in Gannan grassland.The ethanol extract of L.virgaurea is known to possess strong bioactivity.To evaluate the safety of ethanol extract from leaves of L.virgaurea,acute toxicity studies were conducted in mice.All of the tests were based on the 《Toxicological Test Methods of Pesticides for Registration》.The results showed that LD50 of the acute dermal toxicity was greater than 30 g · kg-1,while the LD50 of the acute oral toxicity was 6.983 1 g · kg-1.The ethanol extracts from leaves of L.virgaurea belonged to low-toxic compound,which conformed to the standards of low-toxicity pesticide registration.Based on these results,the ethanol extract may have a potential for exploitation and application as a low-toxicity pesticide.