| 不同处理条件对苜蓿叶蛋白凝聚效果的研究 |
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| 引用本文： | 柳斌,席亚丽,穆峰海,王涛,曹致中.不同处理条件对苜蓿叶蛋白凝聚效果的研究[J].草业科学,2010,27(1):114-118. |
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| 作者姓名： | 柳斌 席亚丽 穆峰海 王涛 曹致中 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州,730070;青岛黄海职业学院电子商务系,山东,青岛266427;河西学院农学系,甘肃,张掖,734000;甘南州畜牧学校,甘肃,合作,747000;兰州大学草地农业科技学院,甘肃,兰州,730020;甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 国家“十一五”科技支撑计划(2006BAD04A04) |
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| 摘 要： | 以现蕾期新鲜苜蓿Medicago sativa为材料,采用温度、pH值单因素处理及温度、加热时间、pH值和NaCl质量分数多因素处理,探讨不同方法对苜蓿叶蛋白凝聚效果的影响。结果表明,在单因素条件下,温度以75℃为宜,pH值4.0对苜蓿叶蛋白产量、叶蛋白纯度和粗蛋白提取率有显著影响。而在多因素条件下,叶蛋白的最适凝聚组合条件为温度85℃,时间2min,pH值4.0,以及NaCl质量分数0.4%。
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| 关 键 词： | 苜蓿 叶蛋白 凝聚条件 |
Study on aggregation effect of alfalfa leaf protein in different treatment conditions |
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| LIU Bin,XI Ya-li,MU Feng-hai,WANG Tao,CAO Zhi-zhong.Study on aggregation effect of alfalfa leaf protein in different treatment conditions[J].Pratacultural Science,2010,27(1):114-118. |
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| Authors: | LIU Bin XI Ya-li MU Feng-hai WANG Tao CAO Zhi-zhong |
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| Institution: | 1.College of Grassland Science;Gansu Agricultural University;Gansu;Lanzhou 730070;China;2.Electron Business Department;Qindao Huanghai Vocational College;Shandong;Qindao 266427;3.Agronomy Department;Hexi College;Zhangye 734000;4.Gannan School of Animal Husbandry;Hezuo 747000;5.College of Pastoral Agriculture Science and Technology;Lanzhou University;Lanzhou 730020;China |
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| Abstract: | This paper took fresh alfalfa at bud stage as material, discussed the effects of different measures on alfalfa leaf protein extraction including temperature, pH and the orthogonal experimental design of four-factors including temperature, heating time, pH and NaCl concentration. The results indicated that in the single factor experiment, the optimum temperature and pH, which affected LPC production, LPC purity and crude protein extraction ratio, were 75 ℃and 4.0, respectively. While in the orthogonal experiment, the optimum combination factors for extracting LPC were that pH was 4.0, temperature was 85 ℃, heating time was 2 min, and NaCl concentration was 0.4%. |
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| Keywords: | alfalfa leaf protein aggregation condition |
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