紫花苜蓿9个盐响应相关基因表达模式

紫花苜蓿(Medicago sativa)是世界上种植面积最大、应用最广泛的豆科牧草。由于其耐盐性中等,其在我国北方地区的产量和种植受到土壤盐渍化的限制。因此提高紫花苜蓿的耐盐性具有重要的科学和生产意义。为此,以龙牧801紫花苜蓿(M.sativa‘Longmu 801’)为试验材料,采用实时荧光定量PCR技术分析了不同浓度NaCl胁迫下9个盐胁迫蛋白质组筛选出的盐响应相关基因的表达模式。结果显示,处理时间和处理浓度对9个基因的相对表达量均有显著性影响,表明这9个基因均在紫花苜蓿盐胁迫应答中发挥着一定作用。处理1h时,G6PI、ABP19a、Trx-h1、PR bet 1、FBPA、6PGDH和ALDH这7个基因的相对表达量在不同NaCl胁迫浓度处理的紫花苜蓿中均显著上调,RRM和GDPD在NaCl处理2h后开始上调。除G6PI基因,其他8个基因在0.4%NaCl处理下相对表达量显著高于0.2%和0.8%NaCl处理。这些基因参与糖代谢、信号转导和胁迫响应。以上结果表明,紫花苜蓿的耐盐性极其复杂,涉及到多基因的表达和代谢通路的调控。研究结果有助于全面研究并了解紫花苜蓿的盐响应相关基因的表达模式,促进紫花苜蓿耐盐分子育种。

Analysis of the expression of nine salt response genes in Medicago sativa

Alfalfa is the most-cultivated and most widely used forage legume in the world.Its moderate salt tol-erance limits its cultivation and production in Northern China because of the soil salinization in this area.There-fore,improving alfalfa salt tolerance is important for both scientific research and commercial production.In this study,we used Medicago sativa 'Longmu 801'as a model to analyze the expression patterns of 9 salt response genes selected for salt stress proteomics using quantitative real-time PCR under various NaCl concentrations. The results showed that time and NaCl concentration had significant effects on the relative expression of all 9 genes,indicating that these 9 genes play roles in alfalfa's salt stress response.The relative expression levels of G6PI,ABP19a,Trx-h1,PR bet 1,FBPA,6PGDH,and ALDH genes were all significantly up-regulated in alfalfa treated with the tested NaCl stress concentrations for 1 h.RRM and GD P D were obviously up-regulated after 2 h of NaCl treatment.Except G6 P I,the relative expression of the remaining 8 genes under 0.4% NaCl stress was much higher than that under 0.2% and 0.8% NaCl stress.These genes are mostly involved in carbo-hydrate metabolism,signal transduction,and stress response.This result demonstrated that alfalfa's salt tol-erance is complicated and is a result of multi-gene expression and the interactions of many metabolic pathways. This result is useful for further research on alfalfa salt response genes and selective breeding of alfalfa using molecular markers.