AM真菌与禾草内生真菌对黑麦草抗旱性的影响

通过温室盆栽试验,从植物生长、养分吸收和抗逆相关酶等方面探究了AM真菌与禾草内生真菌互作对黑麦草(Loliumperenne)抗旱性的影响,以期为充分利用两类微生物来提高植物抗逆性提供理论依据。本研究包括单独接种AM真菌、禾草内生真菌,AM真菌+禾草内生真菌以及无AM真菌和无禾草内生真菌4种处理,黑麦草分别由含有禾草内生真菌(E+)和不含禾草内生真菌(E-)种子建植获得。并于黑麦草生长38 d后,按照土壤最大持水量的15%、30%、45%和60%设置4个水分梯度,共16种处理。结果表明,接种AM真菌与禾草内生真菌显著促进了45%和60%土壤水分条件下黑麦草的生长,促进了其对N、P的吸收,增强了黑麦草体内过氧化物酶活性,降低了丙二醛浓度,表明本研究所用共生微生物仅在较为干旱条件下提高了植物的耐旱性。

Effect of arbuscular mycorrhiza and a grass endophyte on the drought tolerance of perennial ryegrass

We established a pot experiment in a greenhouse to study the effects of arbuscular mycorrhiza(AM) fungus and a grass endophyte on perennial ryegrass drought tolerance,aiming to identify two kinds of symbioticmic roorganisms to improve plant drought stress tolerance.The AM treatments involved inoculation with or without arbuscular mycorrhizal fungus.Seeds of perennial ryegrass infected with a grass endophyte (E+) and without grass endophyte (E-) were used to establish plants with and without a grass endophyte,respectively.After 38 days of growth,ryegrass grown in the above microorganism treatments were exposed to four soil water treatments of 15％,30％,45％,and 60％ of the maximum soil water holding capacity.The mycorrhizalfungus and grass endophyte mostly showed positive effectson plant growth and nutrition uptake in 45％ and 60％ soil water.The two types of symbiotic fungi not onlypromoted the growth of perennial ryegrass and enhanced shoot and root biomass but also promoted plant nitrogen and phosphorus uptake,enhanced peroxidase activity,and reduced malonaldehyde concentrations.Therefore,the two symbionts enhanced plant drought tolerance and reduced (P＜0.05) the damage to perennial ryegrass under semi drought stress.