首页 | 本学科首页   官方微博 | 高级检索  
     检索      

SPF鸡人工感染禽源大肠杆菌、H9亚型禽流感病毒后的体液免疫应答和死亡率分析
引用本文:高璐,胡仁莉,陈祥,盛瑜,刘静,高崧,刘秀梵.SPF鸡人工感染禽源大肠杆菌、H9亚型禽流感病毒后的体液免疫应答和死亡率分析[J].畜牧兽医学报,2006,37(9):899-902.
作者姓名:高璐  胡仁莉  陈祥  盛瑜  刘静  高崧  刘秀梵
作者单位:扬州大学农业部畜禽传染病学重点开放实验室,扬州,225009
基金项目:国家自然科学基金(基金号3047128)
摘    要:SPF鸡经不同顺序、不同时间间隔人工感染MPAIV和E.coli 173株(04)后,对试验鸡的病死率和针对大肠杆菌不同抗原体液免疫应答水平等进行研究。结果表明:各混合感染组试验鸡病死率明显高于单独接种组,且以先接种MPAIV再接种E.coli组死亡率最高;各混合感染组试验鸡针对E.coli OMPs和LPS的抗体水平低于单独感染E.coli组,其中又以先接种MPAIV再接种E.coli组为最低,提示MPAIV和E.coli间存在协同致病作用,这种协同机理可能与因MPAIV的感染导致一定程度的免疫抑制并进而促进了E.coli在体内的定居与繁殖有关。

关 键 词:SPF鸡  大肠杆菌  低致病性禽流感病毒  协同致病  免疫抑制
文章编号:0366-6964(2006)09-0899-04
收稿时间:2005-12-14
修稿时间:2005-12-14

The Mortality Rate and Immune Efficacy of Chickens Inoculated Intratracheally with Lowly Virulent E. coli and/or MPAIV
GAO Lu,HU Ren-li,CHEN Xiang,SHENG Yu,LIU Jing,GAO Song,LIU Xiu-fan.The Mortality Rate and Immune Efficacy of Chickens Inoculated Intratracheally with Lowly Virulent E. coli and/or MPAIV[J].Acta Veterinaria et Zootechnica Sinica,2006,37(9):899-902.
Authors:GAO Lu  HU Ren-li  CHEN Xiang  SHENG Yu  LIU Jing  GAO Song  LIU Xiu-fan
Institution:Animal Infectious Disease Laboratory, Ministry of Agriculture, Yangzhou 225009,China
Abstract:10-day-old specific pathogen free(SPF) chickens were inoculated intratracheally with lowly virulent E.coli and /or mildly pathogenic avian influenza virus(MPAIV).The results were revealed as follows:(1) The mortality rate of chickens inoculated with E.coli and MPAIV simultaneously per bird was 33.3%.Chickens inoculated first with MPAIV and then E.coli at an interval of 48 hours experienced the mortality rate of 66.7%,whereas those inoculated with E.coli and then MPAIV at the same interval exhibited the mortality rate of 16.7%,the group infected with MPAIV alone experienced the mortality rate of 11.1% while the group infected with E.coli alone was found no death.These results suggested that MPAIV might longthen the periods that E.coli colonized in the trachea and lung of inoculated chickens,and it might facilitate E.coli invasion into the respiratory tract of the chickens resulting in more severe pathologic abnormalities.There was a pathogenic synergism between E.coli and MPAIV.(2) An indirect enzyme-linked immunosorbant assay for detection of antibodies to OMPs and an indirect hemagglutination assay for LPS of pathogenic E.coli from chicken origin were used respectively.The antibodies to OMPs and LPS of the birds inoculated first with MPAIV and then E.coli were lower than those of other groups.This results indicated that the synergistic pathopoiesis between E.coli and MPAIV seemed to be related to the immunosuppression exhibited in MPAIV inoculated birds.
Keywords:specific pathogen free chicken  Escherichia coli  mildly pathogenic avian influenza virus  synergistic pathopoiesis effects  immunosuppression
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号