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| 用地高辛标记核酸探针检测鹅细小病毒的研究 | |
| 引用本文： | 布日额,王君伟,吴金花,孙红梅,Ulrich Neumann.用地高辛标记核酸探针检测鹅细小病毒的研究[J].畜牧兽医学报,2004,35(1):102-105. |
| 作者姓名： | 布日额 王君伟 吴金花 孙红梅 Ulrich Neumann |
| 作者单位： | 1. 东北农业大学动物医学院,哈尔滨,150030;内蒙古民族大学动物科技学院,通辽,028042 2. 东北农业大学动物医学院,哈尔滨,150030 3. 内蒙古民族大学动物科技学院,通辽,028042 4. 内蒙古通辽市兽医卫生监督管理所,通辽,028000 5. 汉诺威兽医学院,德国,汉诺威,30559 |
| 基金项目： | 黑龙江省"十五"攻关课题(GB01B50202) |
| 摘 要： | 从带有鹅细小病毒(GPV)NSI基因的重组质粒pMD18T-NS1用限制性内切酶EcoRI和BamH1双酶切回收1880bp大小片段,并制备出地高辛标记的GPV核酸探针。其标记效率达到0．01pg／μl。特异性检测结果表明,该探针能与GPV不同毒株核酸发生特异性杂交,而与对照的DPV、GPPV等病毒的核酸杂交反应均为阴性;敏感性检测结果表明,该探针对GPV的最低检出量为0．0224pg。该探针对不同方法处理的GPV感染病料进行检测,均出现杂交阳性。表明所研制的标记探针用于GPV的检测足可行的。 |
| 关 键 词： | 地高辛标记 核酸探针 检测技术 鹅细小病毒 GPV检测 |
| 文章编号： | 0366-6964(2004)01-0102-04 |
Study on Digoxigenin-Labeled Nucleic Acid Probe for Detection of Goose Parvovirus |
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| Ulrich Neumann.Study on Digoxigenin-Labeled Nucleic Acid Probe for Detection of Goose Parvovirus[J].Acta Veterinaria et Zootechnica Sinica,2004,35(1):102-105. | |
| Authors: | Ulrich Neumann |
| Abstract: | The Goose parvovirus (GPV) 1880 bp NS1 DNA fragment was recuperated from its recombinant plasmid and labeled with Digoxigenin prepared DNA probe for detection of GPV.The hybridization assay result of specificity showed all the DNA of GPV strains were positive,but other nucleotide extracted from DPV,GPPV and allantoic fluid of the nuinoculated embryonating eggs were negertive.The hybridization assay result of sensitivity showed as little as 0.0224 ng of GPV DNA could be detected by DIG-labeled probe.This probe could also hybridizate with GPV neucleotide extracted from the clinical samples which were treated different ways.So this DIG-labeled DNA probe could be used to detect the GPV. |
| Keywords: | Digoxigenin labeled probe Goose parvovirus Detection |
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