戴瑞奶绵羊产奶性状的全基因组关联分析

旨在通过对奶绵羊产奶性状的全基因组关联分析（GWAS），寻找和定位与奶绵羊产奶性状相关的遗传标记和功能基因。本研究以135只戴瑞奶绵羊为试验材料，基于全基因组重测序技术，通过SAMTOOLS进行单核苷酸多态性（SNP）检测，使用PLINK v1.90进行质控，利用GEMMA v 0.98.1的混合线性模型对质控结果进行奶绵羊产奶性状相关的全基因组关联分析。结果表明，有1个SNP与泌乳后90天日均产奶量在全基因组范围内显著相关，8个SNPs达到潜在显著关联，相关的候选基因包括TRNAQ-CUG-2、LOC114117240、ACADL、MYL1、CHD6、SLCO3A1；有2个SNPs与150天日均产奶量达潜在显著关联，相关的候选基因包括PRMT6、RNF180；有2个SNPs与泌乳周期达潜在显著关联，相关的候选基因包括PRMT6、TRNAW-CCA-68、TRNAS-GGA-61。进一步基因功能分析推测，ACADL、SLCO3A1可能是影响奶绵羊产奶性状的候选基因。本研究为奶绵羊产奶性状的分子机制解析提供了一定的基础，为我国奶绵羊新品种培育提供了一定的理论参考。

Genome-wide Association Study of Milk Production Traits in Dairy Meade Sheep

The study aimed to identify and map the genetic markers and functional genes which associated with milk production traits through genome-wide association analysis (GWAS) of milk production traits in Dairy Meade(DM) sheep. In this study, 135 DM sheep were collected and the whole genome resequencing technology was used. The single nucleotide polymorphisms (SNPs) were identified through SAMTOOLS software. The quality control (QC) was performed by Plink v1.90 software. The genome-wide association analysis of milk production traits in DM sheep was performed based on the QC results using the mixed linear model of GEMMA v 0.98.1. The results showed that there was one SNP which was significantly correlated and 8 SNPs were potentially significantly correlated with D90 average daily milk yield trait at genome-wide; The associated candidate genes included TRNAQ-CUG-2, LOC114117240, ACADL, MYL1, CHD6 and SLCO3A1. There were 2 SNPs which were potentially significantly correlated with D150 average daily milk yield trait; The associated candidate genes included PRMT6 and RNF180. There were 2 SNPs which were potentially significantly correlated with lactation period trait; The associated candidate genes included PRMT6, TRNAW-CCA-68 and TRNAS-GGA-61. Further gene functional analysis suggested that ACADL and SLCO3A1 were likely candidate genes which affect milk production traits of DM sheep. This study provides a certain basis for the exploration of milk production traits molecular mechanism in dairy sheep. The result could provide a certain theoretical reference for new dairy sheep breeding in China.