| 鸭腔上囊胚胎及胚后发育期凋亡相关蛋白的免疫组化分析 |
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| 引用本文： | 方静,崔恒敏,崔雪,陈玥.鸭腔上囊胚胎及胚后发育期凋亡相关蛋白的免疫组化分析[J].畜牧兽医学报,2011,42(3). |
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| 作者姓名： | 方静 崔恒敏 崔雪 陈玥 |
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| 作者单位： | 四川农业大学动物医学院,雅安,625000 |
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| 基金项目： | 教育部长江学者和创新团队发展计划(0848) |
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| 摘 要： | 研究天府肉鸭腔上囊胚胎及胚后发育期Bcl-2、Bax、Fas、FasL蛋白的表达。将20只天府肉鸭分为4组,即24天胚龄(E24),胚后3、8、29周龄(P3、P8、P29),采用免疫组化技术。结果显示,Bcl-2、Bax、Fas、FasL在各组滤泡淋巴细胞中均有表达,FasL还表达于滤泡间上皮。滤泡皮质和髓质淋巴细胞Bcl-2阳性率呈递减的变化趋势(髓质P3～8除外);皮质和髓质淋巴细胞Bax阳性率在E24～P8恒定,P29上升;皮质和髓质淋巴细胞Bcl-2/Bax值呈下降趋势,但皮质在P3～8恒定,髓质在P3～8上升;滤泡皮质和髓质淋巴细胞Fas阳性率在E24～P3上升,P3～8下降,P8～29上升;皮质和髓质淋巴细胞FasL阳性率变化规律与Fas相似,但皮质在P3～8恒定。滤泡皮质淋巴细胞Bcl-2阳性率及Bcl-2/Bax值在E24～P8明显高于髓质,在P29则显著低于髓质;皮质淋巴细胞Bax阳性率在E24～P8与髓质无显著差异,在P29则明显高于髓质;皮质淋巴细胞Fas和FasL阳性率在E24～P8明显低于髓质,在P29则显著高于髓质。结果提示,Bcl-2、Bax、Fas、FasL是鸭腔上囊胚胎及胚...
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| 关 键 词： | 天府肉鸭 腔上囊发育 凋亡相关蛋白 Bcl-2 Bax Fas FasL |
Immunohistochemical Analyse of Apoptosis-related Proteins in the Embryonic and Post-embryonic Development of the Duck Bursa of Fabricius |
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| FANG Jing,CUI Heng-min,CUI Xue,CHEN Yue.Immunohistochemical Analyse of Apoptosis-related Proteins in the Embryonic and Post-embryonic Development of the Duck Bursa of Fabricius[J].Acta Veterinaria et Zootechnica Sinica,2011,42(3). |
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| Authors: | FANG Jing CUI Heng-min CUI Xue CHEN Yue |
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| Abstract: | This experiment was conducted to study the expression of Bcl-2,Bax,Fas and FasL proteins during the embryonic and post embryonic development of the Tianfu duck bursa of Fabricius.20 Tianfu ducks were divided into 4 groups as follows: on embryonic day 24(E24),and post-embryonic week 3,8 and 29(P3,P8,P29).Immunohistochemisty technique was used in this study.The results showed that Bcl-2,Bax,Fas and FasL were expressed in the follicle lymphocytes,and FasL was also displayed in the interfollicular epithelia.The... |
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| Keywords: | Tianfu duck development of bursa of Fabricius apoptosis-related proteins Bcl-2 Bax Fas FasL |
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