| 鸡Perilipin1抗血清制备及组织表达特性分析 |
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| 引用本文： | 王彦博,王宁,王丽,王宇祥,李玉茂,李辉.鸡Perilipin1抗血清制备及组织表达特性分析[J].畜牧兽医学报,2011,42(3). |
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| 作者姓名： | 王彦博 王宁 王丽 王宇祥 李玉茂 李辉 |
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| 作者单位： | 东北农业大学动物科技学院,哈尔滨,150030 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2006CB102105),国家重点基础研究发展计划(973计划)项目(2009CB941604); 国家高技术研究发展计划(863)项目(2006AA10A120) |
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| 摘 要： | 通过制备鸡脂滴包被蛋白(Perilipin1)的抗血清,分析鸡Perilipin1的组织表达特性并推测其在脂肪组织脂类代谢过程中发挥的功能。利用RT-PCR的方法扩增鸡Perilipin1基因,并将其构建到含有His标签的表达载体pET-28a上。将阳性重组表达质粒pET-28a/Perilipin1导入大肠杆菌BL21,在IPTG诱导下产生His/Perilipin1融合蛋白,对产生的His/Perilipin1蛋白包涵体进行复性处理,复性后以其为免疫原制备鸡Perilipin1多克隆抗血清。通过ELISA和Western blot的方法检测抗血清的效价及特异性,并利用此抗血清分析鸡Perilipin1在各种组织中的表达特性以及比较其在高、低脂系公鸡间的表达差异。ELISA和Western blot结果表明,本试验获得了效价高、特异性强的鸡Perilipin1抗血清,同时Western blot检测Perilipin1的组织表达特性结果表明,Perilipin1仅在鸡脂肪组织中表达,而在肝脏、十二指肠、胸肌、腿肌、肌胃、心脏、脾脏、肾脏等组织中未检测到表达信号;Perilipin1在肉鸡高脂系脂肪组织中的表...
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| 关 键 词： | 鸡 脂滴包被蛋白 抗血清 表达特性 |
Preparation of Antiserums against Chicken Perilipin1 and Tissue Expression Analyses of Perilipin1 |
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| WANG Yan-bo,WANG Ning,WANG Li,WANG Yu-xiang,LI Yu-mao,LI Hui.Preparation of Antiserums against Chicken Perilipin1 and Tissue Expression Analyses of Perilipin1[J].Acta Veterinaria et Zootechnica Sinica,2011,42(3). |
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| Authors: | WANG Yan-bo WANG Ning WANG Li WANG Yu-xiang LI Yu-mao LI Hui |
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| Institution: | WANG Yan-bo,WANG Ning,WANG Li,WANG Yu-xiang,LI Yu-mao,LI Hui (College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The aim of this study was to analyze the expression characteristics of chicken Perilipin1 in different tissues and the function of Perilipin1 in lipid metabolism by preparing the antiserum against chicken Perilipin1.Chicken Perilipin1 gene was amplified by RT-PCR and inserted into pET-28a vector.The recombinant His/Perilipin1 was expressed in E.coli with IPTG induction and renatured by Protein Refolding Kit.The renatured His/Perilipin1 was used as immunogen to immunize the rabbit for preparing polyclonal an... |
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| Keywords: | chicken Perilipin1 antiserum expression characteristics |
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