抗J亚群禽白血病病毒囊膜糖蛋白特异性单克隆抗体的研制及其特性

J亚群禽白血病病毒（ALV-J）是一种主要感染肉用型鸡的反转录病毒。本研究用表达ALV-J囊膜蛋白基因产物的Sf9细胞免疫Balb/c小鼠，取其脾脏细胞与骨髓瘤细胞NS1进行融合，获得了4株特异性抗ALV-J的单克隆抗体。免疫荧光分析结果表明，3株单克隆抗体仅与所试验的ALV-J毒株反应，而不能与ALV的A、B、C、D和E亚群的毒株反应。有趣的是，有一株单克隆抗体可以与所有试验的外源性ALV毒株反应，但不与内源性的E亚群反应。Western Blot和免疫沉淀试验结果表明，单克隆抗体识别的ALV-J囊膜糖蛋白的分子量为90-94kD，识别未糖基化的囊膜蛋白分子量约为53kD。用这些单克隆抗体能检测出ALV-J病毒感染鸡胚成纤维细胞中的病毒抗原。这些结果提示这些单克隆抗体可用于ALV-J疾病的诊断和流行病学调查。

DEVELOPMENT AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO SUBGROUP J AVIAN LEUKOSIS VIRUS

Avian leukosis virus subgroup J (ALV-J) is a retrovirus which infects meat-type chickens.A panel of monoclonal antibodies (Mabs) to ALV-J was developed by fusion between NS1 and spleen cells from mice immunized with Sf9 cells infected with recombinant baculovirus Hcl-env.Using immunofluorescence assay (IFA),three of four Mabs reacted with ALV-J,but not with subgroups A,B,C,D or E of ALV.Interestingly,one of the four Mabs reacted with all exogenous subgroups of ALV but not with endogenous subgroup E.Western Blot and immunoprecipitation tests showed that molecular weight of ALV-J envelope glycoprotein or unglycosylated envelope protein recognized by Mabs was about 90 kD and 53 kD respectively.Using these Mabs we can detect the ALV-J antigen in CEF infected with ADOL-Hcl.These Mabs can be used for diagnosis and epidemology of ALV-J.