不同日龄猪腹脂中脂肪酸合成酶(FAS)基因表达规律的研究

从猪腹脂中提取总RNA，用RT-PCR扩增脂肪酸合成酶基因（FAS），获得一条206bp的片段，以pGEM-TEasyvector为载体，将该基因片段克隆到大肠杆菌E.coli DH5α，筛选阳性克隆并测序。测序结果表明，得到的片段为FAS基因的部分序列，与GenBank中登录的猪FAS基因（AY183428）494～699之间序列同源性达到100％。以FAS基因片段的克隆为基础，构建了优化的半定量RT-PCR法，以18S rRNA为内标，研究从1日龄到28周龄杜长大腹脂中FAS基因表达的规律。结果显示，从1日龄到28周龄，FAS基因在腹脂mRNA水平呈逐渐升高的趋势；统计分析发现，1日龄与28周龄FAS在腹脂中mRNA水平存在显著性差异（P〈0．05）。

Developmental Gene Expression of Fatty Acid Synthase (FAS) in Abdominal Adipose of Swine

Genome RNA was extracted from adipose of pig and fatty acid synthase(FAS) gene mRNA was amplified using RT-PCR.A DNA fragment about 206bp in length was obtained and the PCR product was cloned into pGEM-T vector.The FAS gene was isolated and sequenced from the positive clones screened.Sequenced analysis suggested that this fragment was partial sequence of FAS cDNA.The gene homology of fragment obtained in this study compared with that of reported FAS cDNA(AY183428)in adipocytes of porcine was 100%.Based on the FAS gene clone,an optimal semi-quantitative RT-PCR method was successfully constructed.Using 18S rRNA as inner control,the developmental gene expression of FAS in abdominal adipose was studied.The results showed that FAS gene expression in abdominal adipose is increased from 1 day to 28 weeks,the difference between 1 day and 28 weeks is significant(P<0.05).