海藻糖对猪精液冷冻保存效果的影响

在传统的Tris-柠檬酸-葡萄糖稀释液基础上，分别添加25％、50％、75％、100％的海藻糖，研究不同浓度海藻糖对猪精液冷冻后精子质量的影响。结果表明，海藻糖相对于对照TCG稀释液能够显著改善和提高猪精液的冷冻效果，其最佳添加浓度为25％，冷冻-解冻后猪精子活力、活率、线粒体活性、质膜完整性以及顶体完整率均显著提高（P〈0．05），分别达到41．38％、46．34％、44．56％、43．51％和64．09％。海藻糖可以明显抑制精子获能，获能处理前精子获能率仅为3．68％，而获能处理后达到41．82％，有利于促进精子获能。精液稀释液中甘油的适宜添加浓度为2％，海藻糖只有与甘油共同作用，才能在冷冻-解冻过程更加有效地保护精子。猪精子活力、活率、线粒体活性、质膜完整率、顶体完整率等之间存在极显著的正相关关系（P〈0．01），而与获能处理前精子的获能率存在显著的负相关关系（P〈0．05）。

Effects of Trehalose on Cryopreservation of Boar Semen

In order to improve boar sperm quality in frozen-thawed process,trehalose was added into the basic extenders-Tris-Citric acid-Glucose.The effects of trehalose on quality of cryopreservation of boar sperm were investigated.Moreover,in order to determine the optimum concentration of trehalose,we designed four different concentrations:25%,50%,75% and 100%(v/v),respectively.The results showed that the effects of the basic extenders supplemented with trehalose on cryoprotection of spermatozoa were better than that of traditional Tris-Citric acidGlucose(TCG) without supplement.We found out that TCG extender with 25% trehalose concentration significantly enhanced motility,viability,mitochondrial activity,membrane integrity and acrosome integrity of boar sperm after freezing and thawing(P<0.05),and they were 41.38%,46.34%,44.56%,43.51% and 64.09%,respectively.Extender containing 25% trehalose could enable some improvement of cryopreservation of boar semen over the currently used TCG medium.Trehalose could decrease distinctly the percentage of spermatozoa capacitated caused by freezing and thawing(3.68%).Capacitation potential was improved significantly with the addition of trehalose in the diluents,especially at 25% concentration(41.82%).The optimal concentration of glycerol in extender was found to be 2%(v/v).Trehalose could improve spermatozoa quality after freeze-thaw process when there was glycerol in the extender.The results of present study indicated that there was a significant positive correlation among sperm motility,viability,mitochondrial activity,membrane integrity and acrosome integrity(P<0.01).However,the percentage of spermatozoa capacitated before capacitation treatment was highly negative correlated with sperm quality index in this study(P<0.05).