二花脸猪和大白猪睾丸IGF-I和IGF-IR基因表达发育变化的研究

为了研究生长轴激素对猪繁殖性能发育的影响,随机选取0、3、20、30、90、120、180日龄纯种二花脸公猪和大白公猪各4头,屠宰并采取睾丸组织样,以18S rRNA为内标,用相对定量RT-PCR法研究睾丸IGF-I和IGF-IRmRNA的表达及发育性变化。结果表明,二花脸猪与大白猪睾丸IG-FI mRNA表达的发育模式在30日龄前完全相同,即随着日龄的增加而呈极显著增加(P<0.01);二花脸猪睾丸IG-FI mRNA相对表达量在30~180日龄无显著变化;大白猪睾丸IGF-I mRNA相对表达量在90日龄有所下降,而在120和180日龄又恢复到30日龄水平。二花脸猪睾丸IG-FI mRNA相对表达量显著高于大白猪(P<0.05)。二花脸猪与大白猪睾丸IGF-IR mRNA表达的发育模式不同。二花脸猪睾丸IG-FIR mRNA相对表达量在90~120日龄呈显著下降趋势(P<0.05);大白猪IG-FIR mRNA相对表达量在0日龄较高,随后显著下降(P<0.05),并在观察期内持续保持较低水平。二花脸猪IG-FIR mRNA相对表达量显著高于大白猪(P<0.05)。睾丸IGF-I mRNA和IG-FIR mRNA相对表达丰度呈极显著正相关(r=0.575,P<0.01)。结论:(1)不同品种猪睾丸IGF-I和IG-FIR mRNA表达具有特定的发育模式;(2)猪睾丸中IGF-I和IG-FIR mRNA的协同表达可能对猪繁殖性能的发育有重要调节作用。

The Developmental Changes of IGF-I and IGF-IR Gene Expression in Testicular of Erhualian and Largewhite Pigs

To understand the relationship between the IGF-I and the porcine fertility development,testicular tissue samples were collected from male Erhualian and Large White pigs at 0,3,20,30,90,120 and 180 days of age respectively.IGF-I and IGF-IR mRNA level were determined by relative quantitative RT-PCR with 18S rRNA as an internal standard.The developmental pattern of testicular IGF-I mRNA expression in Erhualian boars was similar with those in Large White boars from day 0 to 30,which increased with the age of boars.The levels of IGF-I mRNA in Erhualian boars had no significant changes from day 30 to 180,while those in Large White boars showed a temporal drop at day 90 and restored to the level of day 30 thereafter(P<0.05).Overall IGF-I mRNA level was higher in Erhualian boars than in Large White boars(P<0.05).The patterns of testicular IGF-IR mRNA expression in Erhualian and Large White boars were different.The levels of IGF-IR mRNA in Erhualian boars maintained consistent in general throughout the period of investigation,despite a tendency of decrease observed from day 90 to 120(P<0.05).IGF-IR mRNA levels in Large White boars was high at day 0 and declined at day 3(P<0.05),and maintained invariable thereafter.Overall IGF-IR mRNA level was higher in Erhualian boars than in Large White boars(P<0.05).Testicular IGF-I mRNA level was found to be positively correlated with IGF-IR mRNA level(r=0.575,P<0.01).The results suggest that IGF-I and IGF-IR mRNA in porcine testis are coordinately expressed according to breed-specific developmental patterns,and it may play an important role in porcine testis development.