鸡lmbr1基因外显子16的SNP检测和单倍型分析

外显子16是鸡lmbr1基因最大的外显子,本研究进行了该外显子在丝羽乌骨鸡和白洛克肉鸡间的SNP检测和单倍型分析。研究表明,鸡lmbr1外显子16的PCR-SSCP基因型在两个品种间的分布存在明显差异,测序从24个个体中检测到4个变异位点,其中T32C变异在两个品种间存在明显差异,丝羽乌骨鸡均含有32T(纯合的TT或杂合的TC),白洛克肉鸡在T32C位点都为纯合的CC;单倍型分析从24个个体中检测到5种单倍型,丝羽乌骨鸡和白洛克肉鸡的单倍型存在明显的差异,hap1和hap2是丝羽乌骨鸡的特异性单倍型,hap5是白洛克肉鸡的特异性单倍型,hap3和hap4主要存在于白洛克肉鸡中,在丝羽乌骨鸡中的比例极少。

SNP Detection and Haplotype Analysis on Exon 16 of Chicken lmbr1

Exon16 is the biggest exon of chicken lmbr1,here we conduct the SNP detection and haplotype analysis on Silkies and White Plymouth Rock broiler.It shows that the distribution of exon 16 PCR-SSCP genotypes had clear difference between Silkies and White Plymouth Rock broiler,4 variations were detected by sequencing from 24 individuals,the distribution of allele at T32C site had clear difference between Silkies and White Plymouth Rock broiler too,all the individuals of Silky have 32T allele(in homologous TT or heterozygous TC),while all White Plymouth Rock are homologous CC at T32C site.5 haplotypes were detected from 24 individuals,the distribution of haplotypes between two breeds also had clear difference,hap1 and hap2 were the special haplotypes of Silkies,while hap5 was the special haplotype of White Plymouth Rock broiler,the hap3 and hap4 were mainly detected from White Plymouth Rock broiler,which were rare in Silkies.