1例肉鸡肝恶性黑色素瘤的病理学诊断

本研究旨在对临床上发现的1例肉鸡肝黑色素瘤进行病理学诊断。1羽25日龄AA肉鸡精神沉郁、极度消瘦，剖检发现其整个肝呈黑色。HE染色结果显示，病变肝内遍布大小不一、细胞核深染、细胞质内有大量黑色素颗粒沉积的异常细胞团。黑色素瘤诊断标志蛋白SOX 10和HMB-45抗体免疫组化染色均呈阳性。结合病理学检测和免疫组化结果，判定该病鸡肝患有恶性黑色素瘤。试验对病变肝进行胆色素染色和普鲁士蓝染色，结果均呈阴性，提示病变肝并非由胆红素或含铁血黄素淤积所致。综上表明，此例病鸡患有严重的肝恶性黑色素瘤，试验结果对禽类黑色素瘤的诊断与研究有参考价值。

Pathological Diagnosis of a Case of Liver Malignant Melanoma in Broiler Chicken

This research aimed to diagnose a case of liver melanoma that happened in a broiler chicken. A 25-day-old broiler chicken was observed depressed and extremely thin, and the whole liver was black at autopsy. Hematoxylin and eosin (HE) staining results show a large amount of abnormal cells with different sizes, hyperchromatic nuclei and severe melanin granules deposition in the diseased liver. Immunohistochemistry of the liver incubated with both SOX 10 and HMB-45 antibody show positive results. Based on the results of the pathological and immunohistochemical examination, the broiler chicken was diagnosed with serious liver malignant melanoma. Results of both bile pigment and Prussian blue staining were negative, suggesting that the liver lesion was not caused by bilirubin or hemosiderin deposition. In summary, based on the results of differential diagnosis, the broiler chicken was diagnosed with serious liver malignant melanoma. Results of the present study help to provide reference for the diagnosis of avian melanoma in poultry.