鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响

旨在探讨鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响。作者将构建的鸡TGFβ1过表达载体、干扰表达载体以及相应阴性对照转染MDCC-MSB1细胞，然后检测转染后各组细胞鸡TGFβ1的表达水平、细胞增殖能力、细胞周期与凋亡，细胞的迁移与侵袭能力。结果显示，与相应阴性对照相比，转染TGFβ1过表达质粒可显著上调MDCC-MSB1细胞的TGFβ1表达水平，显著抑制MDCC-MSB1细胞的增殖，且使G1期细胞增加、S和G2期细胞减少，同时增加细胞凋亡率，降低细胞的迁移与侵袭能力；转染TGFβ1干扰表达质粒可显著下调MDCC-MSB1细胞的TGFβ1表达水平，显著促进MDCC-MSB1细胞的增殖，G1期细胞减少、S和G2期细胞增加，同时降低细胞凋亡率，增加细胞的迁移与侵袭能力。结果表明，鸡TGFβ1可抑制MDCC-MSB1细胞增殖、迁移与侵袭，促进其凋亡。

Effect of Gallus TGFβ1 on the Proliferation,Apoptosis, Migration and Invasion of MDCC-MSB1 Cells

This study aimed to detect the effect of Gallus TGFβ1 on the biological behavior of MDCC-MSB1 cells. MDCC-MSB1 cells were transiently transfected with Gallus TGFβ1 overexpression vector, interference expression vector, and the corresponding negative control. Then, the expression of Gallus TGFβ1, the cell proliferation, the cell cycle and apoptosis, the migration and invasion of each transfection groups were examined. Results showed that compared with the corresponding control, the MDCC-MSB1 cells transfected with overexpression vector of Gallus TGFβ1 could up-regulate the expression level of TGFβ1, the proliferation of MDCC-MSB1 cells was significantly inhibited, G1 phase cells were increased, S and G2 cells were decreased, the apoptosis rate of the cells was increased, the migration and invasion ability were decreased.However，the MDCC-MSB1 cells transfected with the interference expression vector of TGFβ1 significantly down-regulated the expression level of TGFβ1, cell proliferation was improved，G1 phase cells were decreased, S and G2 cells were increased, the cell apoptosis was decreased, the migration and invasion ability was increased. The results showed that Gallus TGFβ1 could inhibit the proliferation, migration and invasion of MDCC-MSB1 cells, and promote their apoptosis.