|
|||||
|
|
| 牛传染性鼻气管炎病毒内蒙古分离株gB、gC、gD基因的分子克隆与序列分析 | |
| 引用本文： | 胡文兵,关平原,郝瑞峰,赵鹏伟,乌日罕.牛传染性鼻气管炎病毒内蒙古分离株gB、gC、gD基因的分子克隆与序列分析[J].畜牧兽医学报,2012,43(6):965-971. |
| 作者姓名： | 胡文兵 关平原 郝瑞峰 赵鹏伟 乌日罕 |
| 作者单位： | 1. 内蒙古农业大学兽医学院,呼和浩特010018;巴州动物卫生监督所,库尔勒841000 2. 内蒙古农业大学兽医学院,呼和浩特010018;农业部动物疾病临床诊疗技术重点实验室,呼和浩特010018 3. 内蒙古农业大学兽医学院,呼和浩特,010018 4. 内蒙古动物疫病预防控制中心,呼和浩特,010010 |
| 基金项目： | 内蒙古自治区自然科学基金资助项目 |
| 摘 要： | 对牛传染性鼻气管炎病毒(IBRV)内蒙古分离株gB、gC、gD基因进行克隆和序列测定,结果表明IBRV内蒙古分离株gB、gC、gD基因序列分别为2 850、1 723、1 559bp,编码933、508、417个氨基酸组成的完整开放阅读框。序列比较和系统进化树分析结果显示:内蒙古分离株与其他毒株gB、gC、gD基因核苷酸序列的相似性为94.9%～99.9%,其推导的氨基酸相似性在90.3%～99.7%,不同IBRV毒株gB、gC、gD基因在核苷酸和氨基酸水平上高度保守。在系统进化树中内蒙古分离株与瑞典毒株亲缘关系较近,属于同一个分支。 |
| 关 键 词： | 牛传染性鼻气管炎病毒 gB基因 gC基因 gD基因 分子克隆 序列分析 |
Cloning and Sequence Analysis of the gB, gC, gD Genes of IBRV from Inner Mongolia |
|
| HU Wen-bing , GUAN Ping-yuan , HAO Rui-feng , ZHAO Peng-wei , Wurihan.Cloning and Sequence Analysis of the gB, gC, gD Genes of IBRV from Inner Mongolia[J].Acta Veterinaria et Zootechnica Sinica,2012,43(6):965-971. | |
| Authors: | HU Wen-bing GUAN Ping-yuan HAO Rui-feng ZHAO Peng-wei Wurihan |
| Institution: | 1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.Xinjiang Bazhou Animal Health Inspection Institute,Korla 841000,China;3.Center of Prevent and Control of Animal Disease of Inner Mongolia,Hohhot 010010,China;4.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture,Hohhot 010018,China) |
| Abstract: | The gB,gC and gD genes of Inner Mongolia IBRV strain were cloned and sequenced.The results showed that the gB,gC and gD genes were 2 850,1 723 and 1 559 bp,respectively.And the 3 genes contained their corresponding open reading frames(ORF) which encoding 933,508 and 417 amino acids,respectively.The gB,gC and gD genes of Inner Mongolia strain shared 94.9% to 99.9% homology in nucleotide with others strain from GenBank,and shared 90.3% to 99.7% homology in deduced amino acid sequence by the sequences and phylogenetic analysis.The gB,gC and gD genes were highly conserved among IBRV strains.Phylogenetic tree of IBRV revealed that the Inner Mongolia strain was consistently located in the same lineages with Sweden strain. |
| Keywords: | Bovine infectious rhinotracheitis virus gB gene gC gene gD gene molecular cloning sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |