妊娠早期蒙古绵羊子宫内膜enJSRV及IFN-τ基因表达水平的分析

本研究旨在检测内源性绵羊肺腺瘤反转录病毒(enJSRV)与干扰素-τ(IFN-τ)在妊娠早期蒙古绵羊子宫内膜组织的表达以及enJSRV的表达与外周血孕酮水平变化的关系。运用TaqMan实时荧光定量PCR技术和电化学发光法对enJSRV和IFN-τ在妊娠早期蒙古绵羊子宫内膜组织相对表达及孕酮水平进行了测定。实时荧光定量PCR结果显示,enJSRV和IFN-τmRNA在妊娠早期绵羊子宫内膜组织有不同程度的表达。SAS统计学软件分析得出,子宫内膜组织中enJSRV mRNA在妊娠12～14d(交配日为0d)表达较高,2～10、16～30d的表达均低于前者。IFN-τmRNA仅在妊娠12～25d表达,14d达其峰值,30d就不能检出,且差异都极显著(P<0.01)。电化学发光法结果显示,孕酮水平在妊娠2d为0.4ng·mL-1,以后升高,妊娠8～16d维持在8.3ng·mL-1左右,在妊娠19～30d孕酮水平有所下降,30d为2.5ng·mL-1。以上结果提示,子宫内膜组织中enJSRV mRNA的表达与外周血孕酮含量及IFN-τ的变化高度相关,且enJSRV在胎盘的形态发生及生殖生物学方面发挥重要作用。

Analysis of the Expression of enJSRV and IFN-τ in Endometrium of Mongolian Sheep in Early Pregnancy

In order to detect the expression of enJSRV and IFN-τ in endometrial tissue of Mongolian sheep as well as relationship of the expression of enJSRV and the variety of progesterone content in peripheral blood during early pregnancy.TaqMan Real-time Quantify PCR was performed on the relative expression of enJSRV and IFN-τ in uterine endometrium,as well as progesterone content in peripheral blood was detected by electrochemiluminescence analyses.The results demonstrated that enJSRV and IFN-τ mRNA were expressed specifically in uterine endometrium of Mongolian sheep.The mRNA expression level of enJSRV was high on Day 12-14,while lower than the former on Day 2-10 and 16-30 during pregnancy.IFN-τ mRNA was expressed on Day 12-25 of gestation with the maximum value on Day 14,and IFN-τ cannot be detected on Day 30.Electrochemiluminescence results showed the progesterone level was 0.4 ng·mL-1 on Day 2 and rose afterwards on Day 8 of gestation,then kept at about 8.3 ng·mL-1 on Day 8-16 and decreased during Day 19-30 with the value 2.5 ng·mL-1 on Day 30.These results indicated that the expression of enJSRV mRNA in endometrium was highly correlated with changes in peripheral blood progesterone content and IFN-τ.Furthermore,enJSRV plays a significant role during placental morphogenesis and reproductive biology.