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鸡白痢沙门氏菌超广谱β-内酰胺酶基因亚型分析
引用本文:苑丽,潘玉善,吴华,刘建华,胡功政.鸡白痢沙门氏菌超广谱β-内酰胺酶基因亚型分析[J].畜牧兽医学报,2012,43(5):798-802.
作者姓名:苑丽  潘玉善  吴华  刘建华  胡功政
作者单位:河南农业大学牧医工程学院,郑州,450002
基金项目:河南省科技攻关重点项目,河南农业大学博士科研启动资助项目
摘    要:为研究鸡白痢沙门氏菌的耐药表型及产超广谱β-内酰胺酶(ESBLs)的基因亚型,分别采用微量稀释法及PCR扩增、测序比对法对8株分离自河南的鸡白痢沙门氏菌进行耐药表型检测及ESBLs基因亚型分析.结果显示,8株鸡白痢沙门氏菌对氨苄西林、三代头孢、氟苯尼考、恩诺沙星等耐药较为严重,仅对头孢噻呋/舒巴坦(2∶1)、头孢哌酮/他唑巴坦(4∶1)和头孢吡肟高度敏感.PCR检测表明8株受试菌共检出TEM-1(6株)、CTX-M-65(3株)、CTX-M-90(1株)、OXA-1(2株)和OXA-10(1株)5种基因亚型,其中CTX- M-90为国内外首次检出并命名.CTX-M-90的氨基酸序列与CTX-M-14和CTX-M-65相比均仅发生了1处氨基酸变异(80Ala→ Val或275Arg→Ser),故CTX-M-90亚型属于CTX-M-9亚群.结果提示8株鸡白痢沙门氏菌不仅多重耐药严重,而且携带的ESBL基因型复杂.

关 键 词:鸡白痢沙门氏菌  耐药表型  ESBLs  CTX-M-90

Genotype Analysis of Extended-spectrum β-lactamases Produced by Salmonella pullorum
YUAN Li , PAN Yu-shan , WU Hua , LIU Jian-hua , HU Gong-zheng.Genotype Analysis of Extended-spectrum β-lactamases Produced by Salmonella pullorum[J].Acta Veterinaria et Zootechnica Sinica,2012,43(5):798-802.
Authors:YUAN Li  PAN Yu-shan  WU Hua  LIU Jian-hua  HU Gong-zheng
Institution:(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China)
Abstract:The resistant phenotypes and the genotypes of extended-spectrum β-lactamases were detected in Salmonella pullorum by micro-dilution method and PCR-sequencing.The results indicated that the isolates were high resistance to ampicillin,third-generation cephalosporins,florfenicol,and enrofloxacin,although sensitive to cefepime,ceftiofur/sulbactam(2∶1),and cefoperazone/tazobactam(4∶1).Eight isolates harboured five kinds of ESBL genotypes(six TEM-1,three CTX-M-65,one CTX-M-90,two OXA-1 and one OXA-10).To our knowledge,this study is the first to describe and name the CTX-M-90.The amino acid sequence of CTX-M-90 contains only one amino acid alteration,when contrasting to that of CTX-M-14 or CTX-M-65(80Ala→Val,and 275Arg→Ser,respectively).Based on amino acid sequence similarities,CTX-M-90 is classified in the CTX-M-9 subgroup.The results illustrated that eight Salmonella pullorum showed serious multi-resistance and carried complex extended-spectrum β-lactamase genes.
Keywords:Salmonella pullorum  resistant phenotypes  ESBLs  CTX-M-90
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