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牛胚胎性别鉴定与取样胚胎移植应用技术的研究
引用本文:胡明信,黄溢滔.牛胚胎性别鉴定与取样胚胎移植应用技术的研究[J].畜牧兽医学报,1995,26(6):487-495.
作者姓名:胡明信  黄溢滔
作者单位:北京农学院畜牧兽医系,上海医学遗传研究所
摘    要:本研究应用PCR技术扩增牛SRY序列进行奶牛胚胎性别鉴定。经109枚鲜、冻胚的移植,获鲜胚移值妊娠率58.6%(34/58),常规冷冻胚胎移植妊娠率44.4%(12/27),一步细管冷冻解冻胚胎移值妊娠率16.7%(4/24)。犊牛性别验证与SRY鉴定结果均相符合。实验中对胚胎发育时期的划分,胚胎质量评定和胚龄的确定,胚龄与受体发情时间在移植中的关系,胚胎的切割取样,取样胚胎的冷冻进行了研究。

关 键 词:  胚胎  SRY  性别鉴定  移植

SIUDY ON APPLIED TECHNIQUE OF SEXING BOVINE EMBRYOS AND TRANSFER OF BIOPSIED EMBRYOS
Hu Mingxin,Wu Xueqing et al..SIUDY ON APPLIED TECHNIQUE OF SEXING BOVINE EMBRYOS AND TRANSFER OF BIOPSIED EMBRYOS[J].Acta Veterinaria et Zootechnica Sinica,1995,26(6):487-495.
Authors:Hu Mingxin  Wu Xueqing
Abstract:In present study,SRY sequences of the cattle embryo were amplified with theapplication of PCR. A total of 109,including fresh and frozen elnbryos,were transpla-nted.The pregnant rate were 58.6%(34/58) for the fresh,44.4%(12/27)for thefrozen and 16.7%(4/24) for the one step freezing and thawing embryos.The sexualityof calves was consistant with the result of the embryo sex identification. The developm-ent stage of embryo,embryo quality and age,relationship between embryo age andestrus time of recipient,embryo slicing and freezing of sliced embryo were studied.
Keywords:Bovine  Embryo  SRY  Sex diagnosis  Transplanting  
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