猪乙型脑炎血清抗体DIGFA检测方法的建立与应用

本试验建立了一种以固相滤膜为载体，以红色胶体金颗粒标记的免抗猪IgG作为探针，特异性地检测猪流行性乙型脑炎病毒抗体的斑点金免疫渗滤测定法（DIGFA）。与血凝抑制试验（HI）对比检测了54份猪血清临床样本，两法的阳性符合率为71.4%、阴性符合率为65.5%、总符合率为81.4%。本法中抗原抗体通过渗滤在膜上进行反应，数分钟内即可用肉眼观查结果，具有快速，操作简单，敏感性高，特异性强，重复性好，易于判读等优点，特别适合于猪流行性乙型脑炎快速诊断和大规模血清流行病学调查。

The Establishment and Applications of Dot Immuno-Gold Filtration Assay For the Detection of Japanese Encephaltitis Virus Antibody in Swine

Dot Immuno-gold Filtration Assay (DIGFA) with nitrocellulose filter as carrier and the red colloidal gold labeling rabbit-anti-swine IgG as probe was developed for detection of Japanese Encephalitis Virus(JEV) antibody in swine sera,and the optimal working concentration was determined. A comparison was made in parallel with HI test established under the same conditions for the 54 clinical specimens. These two methods had a general coincidence rate of 81.4% ,and the positive and negative rate are 71.4% and 65.5% respectively. The experimental results could be read with the naked eyes within five minutes. This test shows that this method is a rapid, simple, highly sensitive, highly specific and reproducible method. It is an efficient means for rapid diagnosis and epidemiological investigation of JEV.