鸭疫里氏杆菌外膜蛋白免疫原性研究

本研究采用超速离心法提取鸭疫里氏杆菌的外膜蛋白，在电镜下呈典型的双层泡状结构，ＳＤＳ－ＰＡＧＥ电泳分析结果细菌的外膜蛋白的分子量在２１ＫＤ和１５１ＫＤ之间。经Ｗｅｓｔｅｒｎ ｂｌｏｔ检测表明，４４ＫＤ外膜蛋白与免疫鸭血清和自然感染后康复鸭血清出现较强的阳性反应，而感染发病的濒死鸭血清反应则很微弱。

STUDY ON THE IMMUNOGENICITY OF OUTER MEMBRANE PROTEINS OF RIEMERELLA ANATIPESTIFER

s Ultracentrifugationg-Sarkosyl extraction was chosen as the method for isolating the outer membrane proteins(OMPs)of Riemerella anatipestifer(RA).The OMPs showed the typical double round vesicules with negative stain under electronic microscopy.The molecular weight of OMPs range from 23KD to 151KD by SDS-PAGE.The electrophoretic profiles of the 10 isolates of serotype 1 were compared,9 of them were very similar but one isolate differed partially.In the immunoblotting test,the 44KD protein reacted strongly to the sera of ducks immunized with formalin inactivated bacterial antigen and the sera of convalescent ducklings of field infection.The reaction with the sera of dying duckling was very weak. OMPs could induce strong antibody response in ducklings.The protective rate was 100% against the challenge of homologous isolate after the second immunization in laboratory trials.