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| 生长因子EGF、bFGF对猪孤雌胚体外发育的影响 | |
| 引用本文： | 刘吉,潘登科,张莉,牟玉莲,张勇,杨述林,王子荣,冯书堂.生长因子EGF、bFGF对猪孤雌胚体外发育的影响[J].畜牧兽医学报,2007,38(1):36-39. |
| 作者姓名： | 刘吉 潘登科 张莉 牟玉莲 张勇 杨述林 王子荣 冯书堂 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京,100094;新疆农业大学动物科学学院,乌鲁木齐,830052 2. 中国农业科学院北京畜牧兽医研究所,北京,100094 3. 新疆农业大学动物科学学院,乌鲁木齐,830052 |
| 基金项目： | 科技部“863”项目（2003AA205103）;科技部“十五”国家攻关项目（2004BA717B-01） |
| 摘 要： | 在胚胎发育的不同阶段，分别在培养基中添加EGF和bFGF，研究EGF和bFGF对猪孤雌胚体外发育的作用。结果表明：在1细胞阶段添加EGF或bFGF，添加EGF能够显著提高孤雌胚的卵裂率（P〈0．05）；2～4细胞阶段添加EGF和bFGF，添加EGF组和添加bFGF组的囊胚率都显著高于对照组（P〈0．05），而添加bFGF组的囊胚率和囊胚细胞数都略高于对照组和添加EGF组。说明EGF和bFGF有利于猪孤雌胚的体外发育，而且，bF-GF能够通过提高囊胚细胞数而提高猪孤雌胚的质量。 |
| 关 键 词： | 生长因子 猪 体外发育 |
| 文章编号： | 0366-6964（2007）01-0036-04 |
| 修稿时间： | 2006-01-16 |
Effects of EGF or bFGF on the Development of Porcine Parthenogenetic Embryos in vitro |
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| LIU Ji,PAN Deng-ke,ZHANG Li,MU Yu-lian,ZHANG Yong,YANG Shu-lin,WANG Zi-rong,FENG Shu-tang.Effects of EGF or bFGF on the Development of Porcine Parthenogenetic Embryos in vitro[J].Acta Veterinaria et Zootechnica Sinica,2007,38(1):36-39. | |
| Authors: | LIU Ji PAN Deng-ke ZHANG Li MU Yu-lian ZHANG Yong YANG Shu-lin WANG Zi-rong FENG Shu-tang |
| Institution: | 1. Institute o Beij ing f Animal Science, Chinese Academy of Agricultural University, 100094, China ; 2. Department of Animal Science ,Xinjiang Agricultural University, Urumqi 830052, China |
| Abstract: | EGF and bFGF were added into the culture medium in the different stages.We studied the effects of epidermal growth factor or bisac fibroblast growth factor on the development of porcine parthenogenetic embryos in vitro.The results as follows: The addition of EGF significantly enhanced the cleavage rate of porcine parthenogenetic embryos(P<0.05);The addition of EGF or bFGF significantly enhanced the rates of blastocysts formation of 2-4-cell porcine parthenogenetic embryos(P<0.05);Additionally,bFGF had more numbers of blastocysts and higher rates of blastocysts formation than the EGF's and the control.In conclusion,EGF and bFGF are propitious to the development of porcine parthenogenetic embryos in vitro.And bFGF increased the quality of blastocysts by increasing total cell numbers in porcine parthenogenetic embryos. |
| Keywords: | growth factor porcine in vitro culture |
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