| 用抗虫体表膜抗原抗体测定犬细粒棘球绦虫粪抗原 |
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| 引用本文： | 古努尔·吐尔逊,米晓云,张壮志,石保新,吐尔洪·依米提,张旭,巫剑,赵莉,阿曼古丽·马木提,金映红,张文宝.用抗虫体表膜抗原抗体测定犬细粒棘球绦虫粪抗原[J].畜牧兽医学报,2011,42(6):845-850. |
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| 作者姓名： | 古努尔·吐尔逊 米晓云 张壮志 石保新 吐尔洪·依米提 张旭 巫剑 赵莉 阿曼古丽·马木提 金映红 张文宝 |
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| 作者单位： | 1. 新疆畜牧科学院兽医研究所,乌鲁木齐,830000
2. 自治区动物卫生监督所,乌鲁木齐,830063 |
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| 基金项目： | 新疆畜牧科学院青年科研基金，公益性行业(农业)科研专题项目 |
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| 摘 要： | 利用Echinococcus granulosus(Eg)成虫表膜抗原抗体夹心ELISA方法检测犬细粒棘球绦虫感染的粪抗原.首先提取Eg成虫表膜抗原,制备抗Eg成虫表膜抗原的血清,然后用间接ELISA和Western blotting方法检测其抗原抗体反应及免疫原性,用间接免疫组化方法对Eg成虫表膜蛋白进行组织定位.利...
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| 关 键 词： | 细粒棘球绦虫成虫表膜抗原抗体 双抗体夹心ELISA粪抗原 |
Using Antibodies against Tegument Antigens for Copro-ELISA to Detect Echinococcus granulosus in Dogs |
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| Gulnur·Tursun,MI Xiao-yun,ZHANG Zhuang-zhi,SHI Bao-xin,Turgun·Yimit,ZHANG Xu,WU Jian,ZHAO Li,Amangul·mahmut,JIN Ying-hong,ZHANG Wen-bao.Using Antibodies against Tegument Antigens for Copro-ELISA to Detect Echinococcus granulosus in Dogs[J].Acta Veterinaria et Zootechnica Sinica,2011,42(6):845-850. |
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| Authors: | Gulnur·Tursun MI Xiao-yun ZHANG Zhuang-zhi SHI Bao-xin Turgun·Yimit ZHANG Xu WU Jian ZHAO Li Amangul·mahmut JIN Ying-hong ZHANG Wen-bao |
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| Institution: | Gulnur·Tursun1,MI Xiao-yun1,ZHANG Zhuang-zhi1,SHI Bao-xin1,Turgun·Yimit1,ZHANG Xu1,WU Jian1,ZHAO Li1,Amangul·mahmut2,JIN Ying-hong1,ZHANG Wen-bao1 (1.Veterinary Research institute,Xinjiang Academy of Animal Science,Urumqi 830000,China,2.Animal Sanitary Supervision Institute of Xinjiang Uygur Autonomous Region,Urumq 830063,China) |
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| Abstract: | The study was aimed at determining whether antibodies against worm tegument antigens can be used for copro-ELISA to detect Echinococcus granulosus in dogs.In order to produce specific antibodies used for sandwich(copro)-ELISA,we isolated antigens from E.granulosus adult worm tegument.The proteins were used to immunize mice,rabbits and sheep to generate antibodies.ELISA and Western blot analysis were used to identify antigenicity and quality of the serum antibodies.Immunohistochemistry was used to indentify ... |
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| Keywords: | Echinococcus granulosus adult worm tegument Ag copro-ELISA coproantigen |
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