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| 马流感病毒双抗体夹心ELISA检测方法的建立 | |
| 引用本文： | 姬媛媛,郭巍,王晓钧,王征,卢刚,赵立平,李红梅,相文华.马流感病毒双抗体夹心ELISA检测方法的建立[J].畜牧兽医学报,2011,42(5). |
| 作者姓名： | 姬媛媛 郭巍 王晓钧 王征 卢刚 赵立平 李红梅 相文华 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,哈尔滨,150001 2. 东北农业大学生命科学学院,哈尔滨,150030 3. 黑龙江八一农垦大学食品学院,大庆,163319 |
| 基金项目： | 黑龙江省自然科学基金重点项目 |
| 摘 要： | 为建立一种快速、有效的检测马流感病毒(Equine influenza virus,EIV)的方法,以EIV中国分离株A/马/新疆/07(H3N8)制备的多克隆抗体为捕获抗体,原核表达的核蛋白(NP)制备的单克隆抗体为检测抗体,在国内首次建立了检测EIV的双抗体夹心ELISA方法.用该检测方法分别检测EIV、马动脉炎病毒、马疱疹病毒1型、马疱疹病毒4型和马乙型脑炎病毒阳性样品.结果表明,该ELISA方法具有良好的特异性;与常规检测EIV的血凝试验相比,其敏感性是后者的2.5～10倍;同时与H7N7亚型EIV有交叉反应.攻毒试验结果表明该方法可有效检测鼻腔分泌物中的EIV.该方法的建立为EIV的检测及早期防控提供了有效工具. |
| 关 键 词： | 马流感病毒 多克隆抗体 单克隆抗体 双抗体夹心ELISA |
Development of Double Antibody Sandwich ELISA for Detection of Equine Influenza Virus |
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| JI Yuan-yuan,GUO Wei,WANG Xiao-jun,WANG Zheng,LU Gang,ZHAO Li-ping,LI Hong-mei,XIANG Wen-hua.Development of Double Antibody Sandwich ELISA for Detection of Equine Influenza Virus[J].Acta Veterinaria et Zootechnica Sinica,2011,42(5). | |
| Authors: | JI Yuan-yuan GUO Wei WANG Xiao-jun WANG Zheng LU Gang ZHAO Li-ping LI Hong-mei XIANG Wen-hua |
| Institution: | JI Yuan-yuan1,GUO Wei1,WANG Xiao-jun1,WANG Zheng2,LU Gang3,ZHAO Li-ping1,LI Hong-mei1,XIANG Wen-hua1 (1.Division of Livestock Infectious Diseases,State Key Laboratory Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultrual Sciences,Harbin 150001,China,2.College of Life Science,Northeast Agricultural University,Harbin 150030,3.College of Food Science,Heilongjiang August First Land Reclemation University,Daqing 163319,China) |
| Abstract: | To develop a rapid and effective method for Equine influenza virus(EIV) detection,polyclonal antibodies against EIV A/equine/Xinjiang/07 strain and monoclonal antibody against NP of EIV were generated respectively.Then a double antibody sandwich ELISA(DAS-ELISA) was developed.The specificity of the optimized DAS-ELISA was evaluated using EIV,Equine arteritis virus,Equine herpes virus-1,Equine herpes virus-4 and Japanese encephalitis virus,resulting in only EIV specimens yielding a strong signal.Compared wit... |
| Keywords: | Equine influenza virus polyclonal antibodies monoclonal antibody DAS-ELISA |
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