| 中国家驴生长激素基因内含子2的遗传多样性分析 |
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| 引用本文： | 关学敏,朱文进,苏咏梅.中国家驴生长激素基因内含子2的遗传多样性分析[J].畜牧兽医学报,2011,42(6). |
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| 作者姓名： | 关学敏 朱文进 苏咏梅 |
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| 作者单位： | 1. 河北科技师范学院生命科技学院,昌黎,066600
2. 河北科技师范学院动物科技学院,昌黎,066600 |
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| 基金项目： | 河北科技师范学院博士启动基金，青年基金和河北省自然科学基金，河北省教育厅科学研究项目，秦皇岛科学研究与发展项目 |
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| 摘 要： | 为了从分子水平揭示家驴的遗传多样性,本研究以中国7个家驴品种(临县驴、关中驴、新疆驴、广灵驴、淮北驴、德州驴、晋南驴)174个个体为试验动物,利用PCR-SSCP技术研究生长激素(GH)基因第2内含子的遗传多样性并进行序列分析.结果显示:第2内含子表现多态性,174个个体检测到3个单倍型,单倍型比例为1.7%.单倍型多样度以临县驴、淮北驴较高,分别为0.678和0.542.广灵驴与关中驴的单倍型多样度(Haplotype diversity,H)比较接近,分别为0.409和0.462.其次为德州驴和晋南驴,分别为0.355和0.304,新疆驴最低为0.077.对该片段的纯合型分别测序发现,B单倍型在第735位碱基G→C.A单倍型在869位碱基G→T.上述结果首次证实驴GH基因内含子2存在多态性.
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| 关 键 词： | GH基因 PCR-SSCP 遗传多态性 |
Genetic Polymorphisms of the Intron 2 of GH Gene in Chinese Domestic Donkeys |
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| GUAN Xue-min,ZHU Wen-jin,SU Yong-mei.Genetic Polymorphisms of the Intron 2 of GH Gene in Chinese Domestic Donkeys[J].Acta Veterinaria et Zootechnica Sinica,2011,42(6). |
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| Authors: | GUAN Xue-min ZHU Wen-jin SU Yong-mei |
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| Institution: | GUAN Xue-min 1,ZHU Wen-jin 2,SU Yong-mei 2 (1.Department of Life Science,Hebei Normal University of Science & Technology,Changli 066600,China,2.Department of Animal Science,China) |
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| Abstract: | To reveal the genetics diversity of different donkey breeds at molecular level and provide some bases for the breed resource evaluation and utilization of donkeys in China,seven domestic donkey breeds(Linxian,Guanzhong,Xinjiang,Guangling,Huaibei,Dezhou,Jinnan) were used as experimental populations,the genetic variations of GH gene was researched by PCR-SSCP method.The results were as follows: The polymorphic PCR-SSCP sites were in the intron 2 of GH gene.Three haplotypes with the percentage of 1.7% in 174 a... |
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| Keywords: | GH gene PCR-SSCP genetics diversity |
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