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中国家驴mtDNA D-loop 遗传多样性与起源研究
引用本文:葛庆兰,雷初朝,蒋永青,陈宏,张伟,党瑞华,郑惠玲,张爱玲,李天鹏.中国家驴mtDNA D-loop 遗传多样性与起源研究[J].畜牧兽医学报,2007,38(7):641-645.
作者姓名:葛庆兰  雷初朝  蒋永青  陈宏  张伟  党瑞华  郑惠玲  张爱玲  李天鹏
作者单位:1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌,712100
2. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌,712100;徐州师范大学细胞与分子生物学研究所,徐州221116
3. 河南省安阳县畜牧局,安阳,455000
基金项目:西北农林科技大学青年学术骨干人才支持计划(01140304);西北农林科技大学青年科研基金
摘    要:对我国12个家驴品种126个个体(包括引用26个个体)的mtDNA D-loop区399 bp进行分析,共检测到36种单倍型37个多态位点,其单倍型多样度为0.466 7-0.977 8,核苷酸多样度为0.001 2-0.028 5,表明我国家驴的遗传多态性丰富。与3条努比亚野驴、3条索马里野驴和6条亚洲野驴的序列构建NJ系统发育树,首次证明我国家驴的母系起源为非洲野驴中的索马里驴和努比亚驴,亚洲野驴不是中国家驴的祖先。本文还讨论了我国家驴可能的迁徙路线。

关 键 词:中国家驴  mtDNA  D-loop  遗传多样性  起源
文章编号:0336-6964(2007)07-0641-05
修稿时间:2006-07-26

Mitochondrial DNA D-loop Genetic Diversity and Origin of Chinese Domestic Donkeys
GE Qing-lan,LEI Chu-zhao,JIANG Yong-qing,CHEN Hong,HANG Wei,DANG Rui-hua,ZHENG Hui-ling,ZHANG Ai-ling,LI Tian-peng.Mitochondrial DNA D-loop Genetic Diversity and Origin of Chinese Domestic Donkeys[J].Acta Veterinaria et Zootechnica Sinica,2007,38(7):641-645.
Authors:GE Qing-lan  LEI Chu-zhao  JIANG Yong-qing  CHEN Hong  HANG Wei  DANG Rui-hua  ZHENG Hui-ling  ZHANG Ai-ling  LI Tian-peng
Abstract:All 126 mtDNA D-loop sequences 399 bp in length from 12 Chinese domestic donkey breeds were analyzed, including 26 sequences published before. The results revealed 36 different haplotypes with 37 variable sites. The haplotype diversity and the nucleotide diversity were 0. 466 7-0. 977 8 and 0. 001 2-0. 028 5, respectively, indicating high level of genetic diversity in Chinese domestic donkeys. A Neighbor-joining tree constructed using 36 haplotypes of Chinese domestic donkeys, 3 sequences from Nubian wild ass, 3 sequences from Somali wild ass and 6 sequences belonging to Asian wild ass firstly showed that the maternal origin of Chinese domestic donkeys was from Somali and Nubian wild ass in Africa but not from Asian wild ass. We also discussed transport of donkeys among different regions in China.
Keywords:Chinese domestic donkeys  mtDNAD-loop  genetic diversity  origin
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