| 免疫血管活性肠肽调节鸡产蛋和就巢的机理研究 |
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| 引用本文： | 刘颖,陈峰,黄运茂,梁少东,施振旦.免疫血管活性肠肽调节鸡产蛋和就巢的机理研究[J].畜牧兽医学报,2011,42(10). |
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| 作者姓名： | 刘颖 陈峰 黄运茂 梁少东 施振旦 |
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| 作者单位： | 1. 华南农业大学动物科学学院,广州,510642
2. 仲恺农业工程学院生命科学学院,广州,510225 |
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| 基金项目： | 国家自然科学基金，高等学校博士学科点专项科研基金 |
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| 摘 要： | 旨在研究免疫血管活性肠肽(Vasoactive intestinal peptide,VIP)和抑制催乳索(Prolactin,PRL)分泌对鸡产蛋和就巢的影响机制.试验1:对18周龄未开产的泰和鸡,分别在试验d1、d28、d56和d84免疫含VIP羧基端15肽片段的VIP-BSA偶联蛋白,观察对产蛋和就巢的影响.试验2:利用刚开产的23周龄粤黄鸡,在试验d1、d23、d48、d73、d98免疫VIP串联四聚体重组蛋白,观察对产蛋和就巢的影响,并在第98天检测卵泡发育和下丘脑、垂体、F1～F3级LYF颗粒层和膜层相关基因的表达.试验1中免疫VIP组在开产后高峰期(d55～d61)的产蛋率显著低于对照组,但高峰后的(d89～d103)产蛋率显著高于对照组,同时免疫VIP组的就巢发生也要迟于对照组.试验2中,VIP免疫组的产蛋率在试验初高于对照组；在d33将光照从12 h延长至16 h后,2组的产蛋率均快速上升；产蛋高峰后,免疫组产蛋率快速下降并在dS0～d105显著低于对照组.免疫VIP重组蛋白也降低了就巢的发生.2组鸡在d98的大黄卵泡、小黄卵泡和大白卵泡的数量无显著差异.免疫VIP重组蛋白并不影响下丘脑Gn-RH-Ⅰ和VIP、垂体LHβ和FSHβ的mRNA水平,但显著降低了垂体PRL的表达,也显著降低了垂体和下丘脑PRLR的表达.在检测的F1～F3卵泡组织中,免疫VIP重组蛋白也显著降低了F1和F3颗粒层的PRLR mRNA水平,降低了F1～F3颗粒层和膜层的LHR表达量,但对P450scc和StAR的表达量没有影响.两试验结果表明,免疫VIP能够降低PRL的表达和分泌,从而抑制产蛋引发的就巢发生,但是免疫VIP在抑制PRL表达分泌后也会抑制卵泡发育和产蛋,这一机制可能是通过影响卵泡组织LHR的表达而实现的.
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| 关 键 词： | 血管活性肠肽 催乳素 鸡 产蛋 就巢 |
Studies on the Regulation of Egg Laying and Incubation in Hens after Immunization against Vasoactive Intestinal Polypeptide |
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| LIU Ying,CHEN Feng,HUANG Yun-mao,LIANG Shao-dong,SHI Zhen-dan.Studies on the Regulation of Egg Laying and Incubation in Hens after Immunization against Vasoactive Intestinal Polypeptide[J].Acta Veterinaria et Zootechnica Sinica,2011,42(10). |
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| Authors: | LIU Ying CHEN Feng HUANG Yun-mao LIANG Shao-dong SHI Zhen-dan |
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| Institution: | LIU Ying1,CHEN Feng1,HUANG Yun-mao2,LIANG Shao-dong1,SHI Zhen-dan1* (1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China,2.College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China) |
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| Abstract: | This study was conducted to investigate the regulatory mechanisms of egg laying and development of incubation behaviour by immunization against vasoactive intestinal peptide(VIP) in hens.In experiment 1,18-wk old pre-lay Taihe pullets were immunized on d1,d28,d56 and d84 of the experiment,with VIP-BSA conjugate immunogen.In experiment 2,laying 23-wk old Yuehuang hens were immunized on d1,d23,d48,d73 and d98 of the experiment,with tethered 4VIP recombinant protein as immunogen.Tissues of hypothalamus,pituita... |
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| Keywords: | vasoactive inteatinal peptide(VIP) polactin(PRL) chicken laying incubation |
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