| SPMI基因在公母猪生殖道的时空表达特性研究 |
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| 引用本文： | 宋成义,高波,王宵燕,吴晗,周辉云,李碧春,陈国宏,毛九德.SPMI基因在公母猪生殖道的时空表达特性研究[J].畜牧兽医学报,2011,42(11). |
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| 作者姓名： | 宋成义 高波 王宵燕 吴晗 周辉云 李碧春 陈国宏 毛九德 |
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| 作者单位： | 1. 扬州大学动物科学与技术学院,扬州,225009
2. 美国密苏里大学动物科学系,密苏里州哥伦比亚市65211,美国 |
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| 基金项目： | 农业部转基因生物新品种培育重大专项，江苏省自然科学基金 |
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| 摘 要： | 为揭示猪精子运动抑制因子(Seminal plasma motility inhibitor,SPMI)基因在公母猪生殖道的时空表达特性,本研究运用定性RT-PCR分析了SPMI在生殖道的组织表达谱,利用半定量RT-PCR技术分析了SPMI在公猪精囊腺和尿道球腺组织的发育性表达.定性RT-PCR分析结果发现,SPMI基因在精囊腺中的表达丰度最高,在尿道球腺和子宫角中呈中等丰度表达,在前列腺、子宫颈和卵巢中的表达较弱,在其它生殖道组织未检测到mRNA表达.半定量RT-PCR分析结果显示,SPMI基因在公猪精囊腺和尿道球腺中的发育性表达规律相似,都在初生时即启动,表达水平随着日龄的增加不断提高,直至性成熟(150日龄).其中SPMI基因在精囊腺中的表达在60、90和150日龄均有显著提高(P＜0.05)；而在尿道球腺中,SPMI基因的表达在30、90和150日龄均有显著提高(P＜0.05),而60日龄的表达较30日龄时有所提高,但差异不显著(P＞0.05).这些结果提示SPMI基因在公母猪生殖道呈广泛表达,其发育性表达呈日龄依赖性特点,表达水平在初情期至性成熟期有显著提高.
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| 关 键 词： | SPMI基因 生殖道 RT-PCR 时空表达 |
Characteristics of the Spatial and Temporal Expression of SPMI Gene in the Genital Tracts of Boars and Gilts |
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| SONG Cheng-yi,GAO Bo,WANG Xiao-yan,WU Han,ZHOU Hui-yun,LI Bi-chun,CHEN Guo-hong,MAO Jiu-de.Characteristics of the Spatial and Temporal Expression of SPMI Gene in the Genital Tracts of Boars and Gilts[J].Acta Veterinaria et Zootechnica Sinica,2011,42(11). |
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| Authors: | SONG Cheng-yi GAO Bo WANG Xiao-yan WU Han ZHOU Hui-yun LI Bi-chun CHEN Guo-hong MAO Jiu-de |
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| Institution: | SONG Cheng-yi1,GAO Bo1,WANG Xiao-yan1,WU Han1,ZHOU Hui-yun1,LI Bi-chun1,CHEN Guo-hong1,MAO Jiu-de2 (1.College of Animal Science & Technology,Yangzhou University,Yangzhou 225009,China,2.Department of Animal Science,University of Missouri,Columbia MO 65211,USA) |
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| Abstract: | To investigate the spatial and temporal expression profiles of seminal plasma motility inhibitor(SPMI) gene in the genital tracts of boars and gilts,the qualitative RT-PCR was used to detect tissue expression pattern along reproductive tracts and semi-quantitative RT-PCR was applied to determine the developmental expression of SPMI in seminal vesicle and bulbourethral gland in the current study.The qualitative RT-PCR revealed that the expression of SPMI mRNA was highest in seminal vesicle,moderate in bulbou... |
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| Keywords: | SPMI gene genital tracts RT-PCR spatial and temporal expression |
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