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| Dot-ELISA法检测致病性嗜水气单胞菌 | |
| 引用本文： | 任燕,潘子豪,陆承平,姚火春,吴淑勤.Dot-ELISA法检测致病性嗜水气单胞菌[J].畜牧兽医学报,2011,42(10). |
| 作者姓名： | 任燕 潘子豪 陆承平 姚火春 吴淑勤 |
| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广州510380;南京农业大学农业部动物疫病诊断与免疫重点实验室,南京210095 2. 南京农业大学农业部动物疫病诊断与免疫重点实验室,南京,210095 3. 中国水产科学研究院珠江水产研究所,广州,510380 |
| 基金项目： | 现代农业产业技术体系建设专项资金资助，江苏省攻关(三药)项目 |
| 摘 要： | 在鱼类致病性气单胞菌诊断试剂盒的基础上,用斑点酶联免疫吸附试验(Dot-ELISA)检测致病性嗜水气单胞菌(Aeromonas hydrophila,Ah)胞外蛋白酶ECPase54,同时用脱脂奶平板、PCR特异性扩增气溶素基因aer和16S rRNA基因检测72株气单胞菌分离株.结果显示致病性嗜水气单胞菌检测阳性率分别如下:Dot-ELISA法90.3％(65/72)、脱脂奶平板法75％(54/72)、aer基因PCR法94.4％(68/72)、16S rRNA PCR法81.9％(59/72),Dot-ELISA与其他3种方法的符合率分别为79.2％(57/72)、91.6％(66/72)、81.9％(59/72).在ECPase54兔抗血清制备后的2、4、6、12、18个月,用Dot-ELISA检测72株分离株,检测结果重复性好.结果表明Dot-ELISA法敏感、特异、实用,可用于鱼类致病性Ah的临床诊断. |
| 关 键 词： | 嗜水气单胞菌 胞外蛋白酶 斑点酶联免疫吸附试验(Dot-ELISA) 检测 |
Detection of Pathogenic Aeromonas hydrophila by Dot-ELISA |
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| REN Yan,PAN Zi-hao,LU Cheng-ping,YAO Huo-chun,WU Shu-qin.Detection of Pathogenic Aeromonas hydrophila by Dot-ELISA[J].Acta Veterinaria et Zootechnica Sinica,2011,42(10). | |
| Authors: | REN Yan PAN Zi-hao LU Cheng-ping YAO Huo-chun WU Shu-qin |
| Institution: | REN Yan1,2,PAN Zi-hao2,LU Cheng-ping2,YAO Huo-chun2*,WU Shu-qin1 (1.Peal River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.Key Laboratory of Animal Disease Diagnosis and Immunology,Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | On the basis of diagnosis kit for pathogenic Aeromonas,ECPase54 produced by Aeromonas hydrophila(Ah) was detected by Dot-ELISA.On the other hand,the 16S rRNA and aerolysin of pathogenic Aeromonas hydrophila(PAh) were detected with PCR.Furthermore the skimmed milk plate tests were processed.72 Aeromonas isolates were detected with the 4 different methods.The positive rate of 72 isolates was 90.3%(65/72)with Dot-ELISA method,75%(54/72)with skimmed milk plate method,94.4%(68/72)with aer gene PCR method,81.9%(5... |
| Keywords: | Aeromonas hydrophila extracecullar protease(ECPase) Dot-ELISA detect |
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