交感神经对妊娠早期小鼠子宫内膜发育的影响

为了研究交感神经对小鼠妊娠早期(妊娠第1～9天)子宫内膜发育的影响,本试验采用交感神经阻断剂6-羟多巴胺(6-OHDA,100 mg·kg~(-1)体质量)腹腔注射成年雌性昆明小白鼠,损毁外周交感神经.利用放射免疫分析法、组织学和免疫组织化学技术,检测了小鼠妊娠早期血清17β-雌二醇水平、子宫腺发育和子宫内膜细胞增殖的变化.结果显示:(1)在妊娠第1天(E1)、E5和E7 6-OHDA处理组血清中17β-雌二醇的含量分别降低了63.4%(P<0.01)、35.7%(P<0.05)和25.5%(P<0.05),而在E3和E9分别升高了79.3%(P<0.05)和156.7%(P<0.01);(2)6-OHDA处理降低了妊娠早期子宫腺的比例,与同期对照组相比分别下降了37.5%、38.6%、41.8%、61.4%和58.1%(P<0.01);(3)6-OHDA处理降低了妊娠早期子宫内膜细胞的增殖,使E1、E3、E5、E7和E9子宫内膜增殖细胞分别减少了27.2%、30.2%、40.7%、27.2%和27.7%(P<0.01).上述结果表明,交感神经对雌激素的分泌以及妊娠早期子宫组织结构的重建具有重要的调控作用,进而调节动物早期胚胎的着床和发育.

Effect of Sympathetic Nerve on Development of Endometrium in Mice During Early Pregnancy

The objective of the present study was to explore the effect of sympathetic nerve system on development of endometrium in mice during early pregnancy(Embryo day 1-9). The adult fe-male kun-ming mice were treated by intraperitoneal injection of 6-OHDA (100 mg·kg~(-1) body weight), which will damage the sympathetic nerve. After 6-OHDA treatment, the levels of ser-um 17β-estradiol, the development of uterine gland and the proliferation of endometrium cells during early pregnancy were investigated by means of RIA, histology and immunohistochemistry. The results were demonstrated as follows: (1) the levels of 17β-estradiol in 6-OHDA treated group were decreased by 63.4%, 35.7% and 25.5% in Embryo day 1 (E1) (P<0.01), E5 (P< 0.05) and E7 (P<0.05), respectively, but were significantly increased by 79.30% and 156.7% in E3 (P<0.05) and E9 (P<0.01); (2) the proportion of uterine endometrial glands in 6-OH-DA treated group was declined by 37.5%, 38.6%, 41.8%, 61.4% and 58.1% in E1, E3, E5, E7 and E9, respectively (P<0.01); (3) the proliferative rate of endometrial cell during early pregnancy was decreased by 27.2%, 30.2%, 40.7%, 27.2% and 27.7% in E1, E3, E5, E7 and E9, respectively (P<0.01). In conclusion, the sympathetic nerves can regulate the secretion of estrogen and reconstruction of endometrium, further then regulate the animals implantation and development in early pregnancy.