微卫星标记OarAE101和BM143在4个山羊品种中的研究

选择与Booroola羊高繁殖力主效基因Fec^B紧密连锁的2个微卫星标记OarAE101和BM143，分析其在湘东黑山羊、南江黄羊、贵州黑山羊、波尔山羊中的多态分布情况。结果表明微卫星标记OarAE101在4个山羊品种中的等位基因数分别为10、8、6和8，BM143在4个山羊品种中的等位基因数分别为10、8、6和8，OarAE101／BM143在湘东黑山羊、南江黄羊、贵州黑山羊、波尔山羊中的多态信息含量值分别为0．8742／0．8052，0．7969／0．8093，0．7564／0．7462和0．7964／0．7340。湘东黑山羊OarAE101基因型为107bp／111bp基因型所对应的产羔数最小二乘均值最高（4．0头／胎），纯合基因型109bp／109bp、107bp／107bp、119bp／119bp、111bp／111bp和125bp／125bp所对应的产羔数最小二乘平均值较高，分别为2．67、2．5、2．4、2．33和2．25只／胎，故等位基因107、109、111、119和125bp对湘东黑山羊产羔数具有正效应。湘东黑山羊BM143基因型为100bp／106bp和106bp／112bp所对应的产羔数最小二乘平均值达3只／胎，BM143等位基因104、106和110bp与湘东黑山羊产羔数有显著正效应。

Studies of Microsatellite Markers OarAE101 and BM143 in 4 Goat Breeds

2 microsatellite markers OarAE101 and BM143 which were closely linked to the fecundity gene Fec~B in Booroola sheep were analysed for polymorphisms in Xiangdong black goat,Nanjiang brown goat,Guizhou black goat,Boer goat.The number of alleles for OarAE101 was 10,8,6 and 8 in 4 goat breeds.The number of alleles for BM143 was 10,8,6 and 8 in 4 goat breeds.The polymorphic information contents for OarAE101/BM143 in Xiangdong black goat,Nanjiang brown goat,Guizhou black goat,Boer goat were 0.874 2/0.805 2,0.796 9/0.809 3,0.756 4/0.746 2 and 0.796 4/0.734 0,respectively.Least squares means of the litter size for OarAE101 107 bp/111 bp was the best highest(4.0 number born /litter),the least squares means of genotype 109 bp/109 bp,107 bp/107 bp,119 bp /119 bp,111 bp/111 bp,125 bp/125 bp for the litter size were higher,was 2.67,2.5,2.4,2.33 and 2.25 number born /litter respectively,so the allele 107,109,111,119 and 125 bp for the litter size in Xiangdong black goat had positive effects.Least squares mean of litter size for BM143 100bp/106bp and 106 bp /112 bp for the litter size were 3 number born /litter respectively,there were the positive effects among the allele 104,106,110 bp for the litter size of Xiangdong black goat.