印第安纳沙门菌环介导等温扩增检测方法的建立

旨在解决印第安纳沙门菌传统检测方法的缺陷，建立快速、特异、灵敏的分子检测方法。通过比较基因组学方法获取印第安纳沙门菌特异性检测靶标，进一步设计用于环介导等温扩增的引物组。通过对反应条件优化，建立针对印第安纳沙门菌的特异性分子检测方法。结果显示，该检测方法特异性强、耗时短，检测下限为59拷贝·反应^-1。应用该方法对临床分离菌株进行检测，结果不仅与国家标准（GB4789.4—2016）检测结果一致，还可用于自凝菌株检测。本研究建立的方法能实现印第安纳沙门菌的快速检测，将在临床诊断中发挥重要作用。

Establishment of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Salmonella Indiana

The objective of the present study was to establish a molecular method for rapid detection of Salmonella Indiana and overcome the shortcomings of traditional detection method rely on selective isolation,biochemical identification and serological typing.In this study,comparative genomics was used to identify the serospecific gene for S.Indiana,and a set of primers for LAMP was further designed.The specific molecular detection method for S.Indiana was established by optimizing the reaction conditions.The results showed that the method was specific and time-consuming,and the detection limit was 59 copies per reaction.The results of this method are consistent with GB4789.4—2016,and can also be used for the detection of self-coagulating bacteria.The detection method established in this study can rapid detection of S.Indiana,and will play an important role in the clinical diagnosis of this pathogen.