| 山东地方鸡种遗传距离与聚类分析方法比较研究 |
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| 引用本文： | 陈红菊,岳永生,樊新忠,张传生,杜立新.山东地方鸡种遗传距离与聚类分析方法比较研究[J].畜牧兽医学报,2004,35(1):33-36. |
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| 作者姓名： | 陈红菊 岳永生 樊新忠 张传生 杜立新 |
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| 作者单位： | 1. 山东农业大学动物科技学院,泰安,271018
2. 山东农业大学动物科技学院,泰安,271018;中国农业科学院畜牧研究所,北京,100094 |
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| 基金项目： | 山东省农业良种产业化开发畜牧项目~~ |
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| 摘 要： | 选用5个多态性较好的微卫星标记，检测了山东省仅存的5个地方鸡种：寿光鸡、日照麻鸡、莱芜黑鸡、济宁百日鸡、鲁西斗鸡，以及一个外来鸡种——安卡黄鸡和一个外省地方鸡种——广西黄鸡共7个鸡种的遗传多样性。根据测试结果计算了每个等位基因的频率，并以基因频率为基础计算了Nei氏标准遗传距离(Ds)和DA遗传距离，发现日照麻鸡与济宁百日鸡的距离最近，而鲁西斗鸡与其他6个鸡种距离都较远。根据两种遗传距离分别进行了NJ法和UPGMA法聚类，得到4个聚类图。结果表明：DA遗传距离的UPGMA聚类图比较可靠。
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| 关 键 词： | 山东 地方鸡种 遗传距离 聚类分析 的微卫星标记 遗传多样性 |
| 文章编号： | 0366-6964(2004)01-0033-04 |
A Comparative Study of Genetic Distance and Clustering Analysis among Shandong Indigenous Chicken Breeds |
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| CNEN Hong-ju,YUE Yong-sheng,FAN Xin-zhong,ZHANG Chuan-sheng,DU Li-xin.A Comparative Study of Genetic Distance and Clustering Analysis among Shandong Indigenous Chicken Breeds[J].Acta Veterinaria et Zootechnica Sinica,2004,35(1):33-36. |
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| Authors: | CNEN Hong-ju YUE Yong-sheng FAN Xin-zhong ZHANG Chuan-sheng DU Li-xin |
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| Institution: | CNEN Hong-ju~1,YUE Yong-sheng~1,FAN Xin-zhong~1,ZHANG Chuan-sheng~1,DU Li-xin~ |
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| Abstract: | Five microsatellite markers with high polymorphism were selected to test the genetic diversity of seven chicken breeds,five of which were Shandong indigenous ones,Rizhao Pockmarked,Shouguang,Luxi Fighting,Jining Hundred,Laiwu Black,and one was foreign chicken--Anoka Yellow,and another one was Guangxi indigenous chicken--Guangxi Yellow.40 alleles were found,and alleles frequencies and variation within breed were analyzed.Nei's standard genetic distance (D_S,1972)and D_A genetic distance(Nei,et al,1983)among breeds were calculated.NJ tree and UPGMA tree were constructed using D_S and D_A.The result showed that D_A--UPGMA tree was more robust with higher bootstrap values. |
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| Keywords: | Indigenous chicken breeds Genetic distance Clustering analysis |
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