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| 玻璃化冷冻对绵羊卵母细胞中母源基因mRNA表达量的影响 | |
| 引用本文： | 王静,林嘉鹏,石庆华,陈博,汪立芹,赵云程,黄俊成.玻璃化冷冻对绵羊卵母细胞中母源基因mRNA表达量的影响[J].畜牧兽医学报,2011,42(9). |
| 作者姓名： | 王静 林嘉鹏 石庆华 陈博 汪立芹 赵云程 黄俊成 |
| 作者单位： | 1. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,新疆畜牧科学院生物技术中心,乌鲁木齐 830000;新疆农业大学农学院,乌鲁木齐 830052 2. 新疆农业大学农学院,乌鲁木齐,830052 3. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,新疆畜牧科学院生物技术中心,乌鲁木齐 830000 |
| 基金项目： | 新疆自治区自然科学基金(200821182); 新疆自治区科技攻关(200841122); 新疆自治区科技计划项目(200711104); 国家支撑计划(2008BADB2B05-10); 国家“十一五”863计划(2008aa101005); 新疆自治区动物生物技术重点开放实验室开放课题(XJYS0301-2009-01) |
| 摘 要： | 为详细了解玻璃化冷冻对绵羊卵母细胞中母源基因mRNA表达量的影响,分别玻璃化冷冻GV期和IVM 期(18、24 h)绵羊卵母细胞,解冻后进行体外培养.GV期和IVM期卵母细胞经过冷冻-解冻后,卵裂率(10.37％、23.17％、33.07％)均极显著低于对照组(82.96％,P＜0.01),且冷冻-解冻后的GV期卵母细胞卵裂率极显著低于冷冻-解冻后的IVM期卵母细胞(P＜0.01).本试验利用荧光实时定量PCR技术检测4种母源基因:Gdf9(生长分化因子-9)、Zar1(合子阻泄因子)、Mater(胚胎必要的母体抗原)、Dnmt1(DNA甲基化转移酶1)在不同处理后的绵羊卵母细胞中的mRNA含量.结果表明,4个基因的mRNA在GV期的表达量均高于IVM期的卵母细胞(P＜0.01);经玻璃化冷冻处理后,4个基因的mRNA表达量升高,其中GV期含量最高(P＜0.01).结果提示,绵羊GV或IVM期卵母细胞玻璃化冷冻导致母源基因mRNA表达量升高,可能会对胚胎发育产生负面影响. |
| 关 键 词： | 母源基因 玻璃化冷冻 荧光实时定量PCR |
The Influence on mRNA Expression of Maternal Genes in Ovine Oocytes by Vitrified Freezing |
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| WANG Jing,LIN Jia-peng,SHI Qing-hua,CHEN Bo,WANG Li-qin,ZHAO Yun-cheng,HUANG Jun-cheng.The Influence on mRNA Expression of Maternal Genes in Ovine Oocytes by Vitrified Freezing[J].Acta Veterinaria et Zootechnica Sinica,2011,42(9). | |
| Authors: | WANG Jing LIN Jia-peng SHI Qing-hua CHEN Bo WANG Li-qin ZHAO Yun-cheng HUANG Jun-cheng |
| Institution: | WANG Jing1,2,LIN Jia-peng2,SHI Qing-hua1,CHEN Bo1,WANG Li-qin2,ZHAO Yun-cheng2,HUANG Jun-cheng2 (1.Xinjiang Academy of Biotechnological Center,the Key Lab of Livestock Reproduction & Breeding Biotechnology of MOA,Xinjiang Academy of Animal Science,Urumqi 830000,China,2.College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China) |
| Abstract: | To investigate the effect on mRNA expression of maternal genes in ovine oocytes by vitrified freezing,the ovine oocytes at stage GV and IVM(18,24 h) were disposed with the method of vitrified freezing and cultured in vitro under normal conditions after defrosting,and the result showed that the vitrified oocytes at stage GV and IVM both had lower cleavage rate(10.37%,23.17%,33.07%) than that in control group(82.96%,P<0.01).In addition,vitrified oocytes at stage GV had lower cleavage rate than that at stage I... |
| Keywords: | maternal genes vitrified freezing fluorescent real-time quantitative PCR |
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