| 多药耐药相关蛋白2在健康和大肠杆菌感染SD大鼠肝脏和肠组织中的定位及mRNA表达水平 |
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| 引用本文： | 祝玉,苏利娅,王丽平.多药耐药相关蛋白2在健康和大肠杆菌感染SD大鼠肝脏和肠组织中的定位及mRNA表达水平[J].畜牧兽医学报,2011,42(9). |
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| 作者姓名： | 祝玉 苏利娅 王丽平 |
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| 作者单位： | 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 本研究旨在探讨多药耐药相关蛋白2(MRP2)在健康和感染大肠杆菌的SD大鼠肝脏、十二指肠、空肠、回肠和结肠中的分布特点和mRNA表达水平.选用健康成年SD大鼠20只,随机分成健康对照组和大肠杆菌感染组,采用免疫组织化学和荧光定量PCR的方法检测了MRP2在大鼠肝脏和肠组织中的定位与表达.结果显示:MRP2在健康和感染大鼠不同组织中均有分布,主要见于肝脏的胆管面(肝脏毛细胆管的细胞膜)、肝脏门静脉区和各肠段的肠黏膜层,但蛋白表达水平不同.健康组大鼠MRP2在肝脏毛细胆管的细胞膜分布比门静脉区附近广泛,在肝脏毛细胆管的细胞膜和门 静脉区表达均为强阳性;在十二指肠和空肠的表达为强阳性,在回肠和结肠表达为阳性;与健康组相比,MRP2在感染组大鼠的蛋白表达水平均较弱,主要表现为十二指肠和空肠为阳性,回肠和结肠中为弱阳性,肝细胞的胆管面为阳性,肝脏门静脉区附近呈弱阳性.MRP2的mRNA在健康和大肠杆菌感染大鼠的肝脏和肠组织中均有表达,但表达水平也存在差异.与健康组大鼠相比,MRP2 mRNA表达量在大肠杆菌感染组大鼠肝脏和小肠中显著下调(P＜0.05).SD大鼠感染大肠杆菌后可引起肝脏、小肠组织中MRP2在mRNA和蛋白水平的表达下调.
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| 关 键 词： | 药物转运蛋白MRP2 SD大鼠 免疫组织化学 荧光定量PCR |
The Location and Expression Level of Multidrug Resistance Protein 2 in Liver and Intestines of Healthy and E.coli-infected SD Rats |
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| ZHU Yu,SU Li-ya,WANG Li-ping.The Location and Expression Level of Multidrug Resistance Protein 2 in Liver and Intestines of Healthy and E.coli-infected SD Rats[J].Acta Veterinaria et Zootechnica Sinica,2011,42(9). |
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| Authors: | ZHU Yu SU Li-ya WANG Li-ping |
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| Institution: | ZHU Yu,SU Li-ya,WANG Li-ping (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The study was conducted to investigate the level difference of multidrug resistance protein 2(MRP2) proteins and mRNA expressions in the liver,duodenum,jejunum,ileum and colon of the healthy and E.coli-infected rats.Twenty healthy adult SD rats were randomly divided into control group(administered with sterile pathogen-free physiology saline) and E.coli-infected group.The immunohistochemical and real time RT-PCR methods were used in this study.The results showed that MRP2 was expressed in all tissues of hea... |
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| Keywords: | drug transporter MRP2 SD rats immunohistochemistry real time RT-PCR |
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